Assessment of antibiotic resistance patterns in two potential probiotic bacteria, Lactococcus lactis subsp. cremoris NABRII64 and Lactococcus lactis subsp. cremoris NABRII66 isolated from rainbow trout (Oncorhynchus mykiss) intestine

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Mortezaei Faezeh; Royan Maryam; Pourebrahim Moslem; BABAKHANI ARIA

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Journal: AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY Year:2020 | Volume:8 | Issue:1 Page(s): 1-18

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Title

Assessment of antibiotic resistance patterns in two potential probiotic bacteria, Lactococcus lactis subsp. cremoris NABRII64 and Lactococcus lactis subsp. cremoris NABRII66 isolated from rainbow trout (Oncorhynchus mykiss) intestine

Author(s)

Mortezaei Faezeh | Royan Maryam | Pourebrahim Moslem | BABAKHANI ARIA | Issue Writer Certificate

Keywords

Rainbow TroutQ1

Antibiotic ResistanceQ2

Tetracycline ResistanceQ1

Plasmid DNAQ1

Lactococcus lactisQ1

Abstract

This study aimed to investigate the phenotypic and genotypic patterns of Antibiotic Resistance in two potential probiotic lactic acid bacteria, Lactococcus lactis subsp. cremoris (NABRII64 and NABRII66) isolated from the Rainbow Trout intestine. The phenotypic susceptibility pattern of the strains was studied based on the Minimum Inhibitory Concentration (MIC) of eight most commonly used antibiotics in medicine and veterinary including ampicillin, kanamycin, gentamicin, streptomycin, erythromycin, clindamycin, tetracycline and chloramphenicol. After comparing the MICs with standard values recommended by European Food Safety Authority (EFSA), the nature of the phenotypic resistance observed in bacterial strains was investigated by polymerase chain reaction (PCR) through Plasmid DNA extraction. The results of phenotypic evaluation indicated the Tetracycline Resistance in both bacterial strains (MIC<4mg/L). Genotyping of Antibiotic Resistance genes including tet (S), tet (L), tet (M), tet (O), tet (W) and tet (K) indicated the presence of tet (S) and tet (M) resistance genes in Plasmid DNA of both bacterial strains. These results exhibited the acquired resistance and the presence of two Tetracycline Resistance genes in the Plasmid DNA of two bacterial strains, NABRII64 and NABRII66. However, further studies are required to understand the nature of the acquired resistance mechanism in the future.

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APA: Copy

Mortezaei, Faezeh, Royan, Maryam, Pourebrahim, Moslem, & BABAKHANI, ARIA. (2020). Assessment of antibiotic resistance patterns in two potential probiotic bacteria, Lactococcus lactis subsp. cremoris NABRII64 and Lactococcus lactis subsp. cremoris NABRII66 isolated from rainbow trout (Oncorhynchus mykiss) intestine. AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY, 8(1 ), 1-18. SID. https://sid.ir/paper/394487/en

Vancouver: Copy

Mortezaei Faezeh, Royan Maryam, Pourebrahim Moslem, BABAKHANI ARIA. Assessment of antibiotic resistance patterns in two potential probiotic bacteria, Lactococcus lactis subsp. cremoris NABRII64 and Lactococcus lactis subsp. cremoris NABRII66 isolated from rainbow trout (Oncorhynchus mykiss) intestine. AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY[Internet]. 2020;8(1 ):1-18. Available from: https://sid.ir/paper/394487/en

IEEE: Copy

Faezeh Mortezaei, Maryam Royan, Moslem Pourebrahim, and ARIA BABAKHANI, “Assessment of antibiotic resistance patterns in two potential probiotic bacteria, Lactococcus lactis subsp. cremoris NABRII64 and Lactococcus lactis subsp. cremoris NABRII66 isolated from rainbow trout (Oncorhynchus mykiss) intestine,” AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY, vol. 8, no. 1 , pp. 1–18, 2020, [Online]. Available: https://sid.ir/paper/394487/en

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