Effect of three administration routes of streptococosis/lactococosis Bacterin on specific immunity and expression of IgM and IL-6 genes in rainbow trout

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Alishahi Mojtaba; Halimi Mostafa; GHORBANPOUR MASOUD; TABANDEH MOHAMMAD REZA

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Journal: AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY Year:2020 | Volume:8 | Issue:1 Page(s): 123-146

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Title

Effect of three administration routes of streptococosis/lactococosis Bacterin on specific immunity and expression of IgM and IL-6 genes in rainbow trout

Author(s)

Alishahi Mojtaba | Halimi Mostafa | GHORBANPOUR MASOUD | TABANDEH MOHAMMAD REZA | Issue Writer Certificate

Keywords

Streptococosis/Lactococosis BactrinQ1

Administration RoutesQ1

Vaccine EfficacyQ1

IL-6Q1

IgMQ1

Rainbow TroutQ2

Abstract

In this study the effect of streptococosis/lactococosis bacterin administrated via oral, bath and intraperitoneal routes on the expression of two immune related genes (IgM and IL-6) was investigated. 600 fish (14± 2. 1g) were randomly divided into 4 groups in triplicates. Group 1 was vaccinated via oral route using 10 8 CFU bacterial cell (Streptococcus iniae and Lactococcus garvieae) in each gram of food. Group 2 was injected with 0. 1mL of 10 9 CFU/mL bacterin. Group 3 was vaccinated via immersion route for 2 minutes in 10 9 CFU/mL. Group 4 was none immunized group without vaccination. Fish were reared for two months in a similar situation. At days of 0, 20, 40 and 60, antibody titer against both bacteria was performed by ELISA method and expression of IL-6 and IgM genes were evaluated through qRT-PCR method. Then fishes were exposed to S. iniae and L. garvieae separately at the end of the experiment and the rate of mortality was compared among the groups. The results showed that the rate of mortality in exposed fish to S. iniae in injection, oral, immersion and unvaccinated routs were 20± 10, 56. 3± 10, 50± 5. 67 and 90± 3. 67 whereas in exposed fish to L. garvieae were 26. 67± 5. 77, 57. 7± 10, 43. 33± 5. 67 and 90± 4. 63 respectively. The antibody titer in the vaccine injection group was increased compared to the control (P<0. 05). Expression of IL-6 and IgM in vaccinated groups was significantly increased compared to control group (P<0. 05) with the highest rate in the injection group. It can be concluded that although injection route causes the best protection, antibody titer and expression of immune genes, oral and immersion routes could also induce relative protection and immunogenicity in Rainbow Trout and each route possesses some priority over other routes then further research on efficacy improvement of each route can be recommended.

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APA: Copy

Alishahi, Mojtaba, Halimi, Mostafa, GHORBANPOUR, MASOUD, & TABANDEH, MOHAMMAD REZA. (2020). Effect of three administration routes of streptococosis/lactococosis Bacterin on specific immunity and expression of IgM and IL-6 genes in rainbow trout. AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY, 8(1 ), 123-146. SID. https://sid.ir/paper/394922/en

Vancouver: Copy

Alishahi Mojtaba, Halimi Mostafa, GHORBANPOUR MASOUD, TABANDEH MOHAMMAD REZA. Effect of three administration routes of streptococosis/lactococosis Bacterin on specific immunity and expression of IgM and IL-6 genes in rainbow trout. AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY[Internet]. 2020;8(1 ):123-146. Available from: https://sid.ir/paper/394922/en

IEEE: Copy

Mojtaba Alishahi, Mostafa Halimi, MASOUD GHORBANPOUR, and MOHAMMAD REZA TABANDEH, “Effect of three administration routes of streptococosis/lactococosis Bacterin on specific immunity and expression of IgM and IL-6 genes in rainbow trout,” AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY, vol. 8, no. 1 , pp. 123–146, 2020, [Online]. Available: https://sid.ir/paper/394922/en

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