Correlation between Promoter Hypermethylation and Expression Profiles of P15INK4b and P16INK4a Genes on Development of Pterygium Disease

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Dianat Tahereh; Aminifard Mohammad Naiem; Arish Mohammad; Sangtrash Mohammad Hussein; Poyandeh Robab

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Journal: Journal of Epigenetics Year:2020 | Volume:2 | Issue:1 Page(s): 27-31

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Title

Correlation between Promoter Hypermethylation and Expression Profiles of P15INK4b and P16INK4a Genes on Development of Pterygium Disease

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Keywords

Pterygium

Hypermethylation

Expression

P15INK4b Gene

P16INK4a Gene

Abstract

Background: It is not thoroughly clear that what is the exact etiology of Pterygium. Recently, it has been illustrated that Pterygium is a benign and destructive condition. The aim of this study was to examine the correlation between promoter Hypermethylation of P15INK4b and P16INK4a Genes on progress of Pterygium. Materials and methods: We extracted DNA from 81 primary Pterygium and 75 normal conjunctiva tissues. Methylation specific polymerase chain reaction (MSP) technique was used to analyze of promoter Hypermethylation of P15INK4b and P16INK4a Genes. The Expression levels of these genes were also assessed in mRNA from 23 Pterygium and 18 normal conjunctiva tissue samples using real-time quantitative reverse transcriptase PCR. Results: The frequency of methylation for P15INK4b was 97. 5% and 72% among cases and controls respectively. P16INK4 gene methylation at promoter was 69. 1% and 33. 3% for Pterygium and normal conjunctiva tissue respectively. A statistically significant relationship was found for methylation of P15INK4b and P16INK4a Genes between cases and controls (P<0. 0001). The relative Expression of P16INK4a Gene also in Pterygium tissues was significantly different in comparison to conjunctiva tissues of healthy controls (P<0. 0001). But, it was not significant for P15INK4b. Conclusion: These data suggest that reduced Expression of P15INK4b and especially P16INK4a Genes through promoter Hypermethylation of these two critical genes in cell cycle between cases and controls is probably an early and common epigenetic alteration in Pterygium. More investigations are required to be performed to analyze of epigenetic variations effect on the progression of Pterygium in different stages.

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APA: Copy

Dianat, Tahereh, Aminifard, Mohammad Naiem, Arish, Mohammad, Sangtrash, Mohammad Hussein, & Poyandeh, Robab. (2020). Correlation between Promoter Hypermethylation and Expression Profiles of P15INK4b and P16INK4a Genes on Development of Pterygium Disease. JOURNAL OF EPIGENETICS, 2(1 ), 27-31. SID. https://sid.ir/paper/397351/en

Vancouver: Copy

Dianat Tahereh, Aminifard Mohammad Naiem, Arish Mohammad, Sangtrash Mohammad Hussein, Poyandeh Robab. Correlation between Promoter Hypermethylation and Expression Profiles of P15INK4b and P16INK4a Genes on Development of Pterygium Disease. JOURNAL OF EPIGENETICS[Internet]. 2020;2(1 ):27-31. Available from: https://sid.ir/paper/397351/en

IEEE: Copy

Tahereh Dianat, Mohammad Naiem Aminifard, Mohammad Arish, Mohammad Hussein Sangtrash, and Robab Poyandeh, “Correlation between Promoter Hypermethylation and Expression Profiles of P15INK4b and P16INK4a Genes on Development of Pterygium Disease,” JOURNAL OF EPIGENETICS, vol. 2, no. 1 , pp. 27–31, 2020, [Online]. Available: https://sid.ir/paper/397351/en

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