Cloning and Expression of β (1-3)(1-4) Glucanase Gene in E. coli for Production as Animal Feed Supplement

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AFSHIN H.; MIRAKHORLI N.; SAFFAR B.; KHAJALI F.

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Journal: Journal of Molecular and Cellular Researches Year:2020 | Volume:33 | Issue:1 Page(s): 64-74

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Title

Cloning and Expression of β (1-3)(1-4) Glucanase Gene in E. coli for Production as Animal Feed Supplement

Author(s)

AFSHIN H. | MIRAKHORLI N. | SAFFAR B. | KHAJALI F. | Issue Writer Certificate

Keywords

Recombinant enzymeQ1

lichenaseQ1

Poultry diets supplementQ1

Abstract

β-Glucans (beta-glucans) form a natural component of the cell walls of bacteria, fungi, yeast, and cereals such as oat and barley. Glucanases are enzymes that break down a glucan. β-glucans are chains of D-glucose polysaccharides linked by β-type glycosidic bonds. The purpose of this study is to express the lechinase gene in bacteria to produce Recombinant enzyme as a feed supplement in poultry diets. In this study, LicBM2 gene, isolated from Clostridium thermocellum, encodes thermostable lichenase enzyme was cloned in expression vector pET22b (+) and E. Coli bacteria strain BL21. Bacterial β 1, 3– 1, 4-glucanases (EC 3. 2. 1. 73; lichenase) specifically cleave β-1, 4-glycosidic linkage adjacent to 3-O-substituted glucopyranose residues. Gene expression was confirmed using SDS-PAGE techniques. The enzyme activity of recombinant protein and reducing glucan in barly were measured by DNS method. The optimum temperature for recombinant protein production was 55 ° C and the maximum enzyme activity was obtain within 4 hours after inducing in pH = 8. Using barlay in poultry diet is more economical. But the significant amounts of beta-glucans in barlay cause more problems. The Recombinant enzyme produced in this study can be used as a feed supplement for hydrolyzation of barley β-glucan to replace of corn by barley in poultry diets.

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APA: Copy

AFSHIN, H., MIRAKHORLI, N., SAFFAR, B., & KHAJALI, F.. (2020). Cloning and Expression of β (1-3)(1-4) Glucanase Gene in E. coli for Production as Animal Feed Supplement. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 33(1 ), 64-74. SID. https://sid.ir/paper/399955/en

Vancouver: Copy

AFSHIN H., MIRAKHORLI N., SAFFAR B., KHAJALI F.. Cloning and Expression of β (1-3)(1-4) Glucanase Gene in E. coli for Production as Animal Feed Supplement. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2020;33(1 ):64-74. Available from: https://sid.ir/paper/399955/en

IEEE: Copy

H. AFSHIN, N. MIRAKHORLI, B. SAFFAR, and F. KHAJALI, “Cloning and Expression of β (1-3)(1-4) Glucanase Gene in E. coli for Production as Animal Feed Supplement,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 33, no. 1 , pp. 64–74, 2020, [Online]. Available: https://sid.ir/paper/399955/en

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