Evaluating the Safety of Indigenous Enterococcus species isolated from dairy products and Breast milk by Biochemical and Molecular Methods

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Khatibi Mahzad; Vaseji Narges; Salehi Behzad; Mojgani Nahid

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Journal: Animal Science Journal Year:2018 | Volume:31 | Issue:120 Page(s): 89-102

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Title

Evaluating the Safety of Indigenous Enterococcus species isolated from dairy products and Breast milk by Biochemical and Molecular Methods

Author(s)

Khatibi Mahzad | Vaseji Narges | Salehi Behzad | Mojgani Nahid | Issue Writer Certificate

Keywords

ProbioticQ1

enterococcusQ1

16S rRNAQ1

virulence genesQ1

vancomycin resistanceQ1

Abstract

enterococcus plays an important role in various industries. Some Enterococci isolated from dairy and breast milk has been reported as Probiotics. However, these species selected as starter cultures or as Probiotics should be evaluated for their safety and the absence of different virulence traits including virulence genes and antibiotic resistance. The aim of this study was to determine the safety of locally isolated enterococcus species biochemically and targeting essential virulence genes by PCR method. A number of suspected enterococcus species isolated from ewe milk, traditional cheese and mothers' milk were identified to genus level by phenotypic characteristics and 16SrRNA sequencing. The selected isolates were screened for their Probiotic properties by determining their acid and bile resistance, antibacterial activity against a number of pathogens and antibiotic resistance. Phenotypic virulence parameters including lipase, DNAse and hemolysis of red blood cells, was determined. The presence or absence of a number of virulence genes including asa1, hyl, esp, agg, gelE, cylA, cylB, cylM, efaAfm, efaAfs and vancomycin resistance genes including vanA, vanH, vanR and vanY was evaluated. The results of this study determined the Probiotic properties of some of these species. Four isolates showed acid and bile salt tolerance and showed significant antimicrobial effects. All isolates from Breast milk, lacked virulence genes. In virulance phenotypic experiments, all strains were alpha hemolytic. All strains were susceptible to Tetracycline, Cephalothin Amoxicillin, Cefazolin and Doxycycline and all of them except the TA00154 strain, were resistant to Nalidixic acid and Trimethoprim sulfamethoxazole.

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APA: Copy

Khatibi, Mahzad, Vaseji, Narges, Salehi, Behzad, & Mojgani, Nahid. (2018). Evaluating the Safety of Indigenous Enterococcus species isolated from dairy products and Breast milk by Biochemical and Molecular Methods. ANIMAL SCIENCES JOURNAL (PAJOUHESH & SAZANDEGI), 31(120 ), 89-102. SID. https://sid.ir/paper/407114/en

Vancouver: Copy

Khatibi Mahzad, Vaseji Narges, Salehi Behzad, Mojgani Nahid. Evaluating the Safety of Indigenous Enterococcus species isolated from dairy products and Breast milk by Biochemical and Molecular Methods. ANIMAL SCIENCES JOURNAL (PAJOUHESH & SAZANDEGI)[Internet]. 2018;31(120 ):89-102. Available from: https://sid.ir/paper/407114/en

IEEE: Copy

Mahzad Khatibi, Narges Vaseji, Behzad Salehi, and Nahid Mojgani, “Evaluating the Safety of Indigenous Enterococcus species isolated from dairy products and Breast milk by Biochemical and Molecular Methods,” ANIMAL SCIENCES JOURNAL (PAJOUHESH & SAZANDEGI), vol. 31, no. 120 , pp. 89–102, 2018, [Online]. Available: https://sid.ir/paper/407114/en

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