Evaluation of Immunogenicity of PLGA – Proteus mirabilis Detoxified Lipopolysaccharide Conjugate Against Urinary Tract Infection in Mice

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SAEIDI E.; SHAPOURI R.; RAHNEMA M.; SALOUTI M.; Nasserian J.

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Journal: JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES) Year:2020 | Volume:13 | Issue:4 (51) Page(s): 99-115

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Title

Evaluation of Immunogenicity of PLGA – Proteus mirabilis Detoxified Lipopolysaccharide Conjugate Against Urinary Tract Infection in Mice

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Keywords

Proteus mirabilisQ1

Co-glycolic poly lactic acidQ1

Lipopolysaccharide conjugationQ1

Nano-vaccineQ1

Abstract

Inroduction & Objective: Spermatogonial stem cells (SSCs) as adult stem cells are crucial for spermatogenesis. Electromagnetic fields (EMF) leads to biological activity disruption of these cells and spermatogenesis. Antioxidants like vitamin C can reduce the damage caused by EMF through oxidative stress reduction. Recent studies also reported the key role of Sertoli cell paracrine signaling in regulating the maintenance and differentiation of SSCs. Thus, we examined and compared the effect of vitamin C and exosomes derived from Sertoli cells on damage induced by EMF in SSCs. Materials and Methods: In the present study, after the massive cultivation of Proteus mirabilis, its LPS was extracted, purified and detoxified and ultimately conjugated with PLGA nanoparticle. This conjugation was confirmed with Zeta Sizer, FTIR and AFM devices. Vaccination was injected intramuscularly, 3 times in two weeks, to 4 groups of 3 mice treated with normal saline (control group), LPS, PLGA and PLGA-LPS conjugate. To perform the challenge test, mice were anesthetized and operated with ethical rights in sterile conditions and 6*107 CFU concentrations of Proteus mirabilis bacteria was injected into their bladder. After one week of injection, their bladder was removed and suspended in a salt buffer. Results: The results of the challenge test confirmed the success of the PLGA-LPS conjugate in comparison with other groups. In other words, the control and PLGA groups had the highest growth rates, and the average number of colonies was significantly higher than all of the groups. And the number of colonies grown in groups were LPS-PLGA(6. 2 *10 CFU/ml), LPS(3. 6 *103 CFU/ml), PLGA (91. 3*102 CFU/ml) and NS(98 * 102 CFU/ml). According to one-way ANOVA, which was done by SPSS software, there was a significant difference between all groups (P <0. 05). the PLGA immunogenicity was significantly different with the control group. Conclusion: Therefore, it can be concluded that the PLGA-LPS conjugate has an appropriate immunity and, by examining its non-toxicity in pathologic studies, can be a candidate of nanovaccine for prevention of urinary tract infection caused by Proteus migrailitis.

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APA: Copy

SAEIDI, E., SHAPOURI, R., RAHNEMA, M., SALOUTI, M., & Nasserian, J.. (2020). Evaluation of Immunogenicity of PLGA – Proteus mirabilis Detoxified Lipopolysaccharide Conjugate Against Urinary Tract Infection in Mice. JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES), 13(4 (51) ), 99-115. SID. https://sid.ir/paper/411403/en

Vancouver: Copy

SAEIDI E., SHAPOURI R., RAHNEMA M., SALOUTI M., Nasserian J.. Evaluation of Immunogenicity of PLGA – Proteus mirabilis Detoxified Lipopolysaccharide Conjugate Against Urinary Tract Infection in Mice. JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES)[Internet]. 2020;13(4 (51) ):99-115. Available from: https://sid.ir/paper/411403/en

IEEE: Copy

E. SAEIDI, R. SHAPOURI, M. RAHNEMA, M. SALOUTI, and J. Nasserian, “Evaluation of Immunogenicity of PLGA – Proteus mirabilis Detoxified Lipopolysaccharide Conjugate Against Urinary Tract Infection in Mice,” JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES), vol. 13, no. 4 (51) , pp. 99–115, 2020, [Online]. Available: https://sid.ir/paper/411403/en

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