EFFICIENCY OF SEROLOGICAL TECHNIQUES FOR DETECTING SINGLE AND MULTIPLE INFECTIONS OF MAJOR POTATO VIRUSES IN THE SEED PRODUCTION SYSTEM

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

EMAMI MEYBODI D.; MOZAFARI J.; BABAEIAN N.A.; RAHIMIAN H.A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF PLANT PATHOLOGY Year:2007 | Volume:43 | Issue:3 (171) Page(s): 338-346

Download Full-Text

Persian Verion

View:

2,195

Download:

Cites:

Information Journal Paper

Title

EFFICIENCY OF SEROLOGICAL TECHNIQUES FOR DETECTING SINGLE AND MULTIPLE INFECTIONS OF MAJOR POTATO VIRUSES IN THE SEED PRODUCTION SYSTEM

Author(s)

EMAMI MEYBODI D. | MOZAFARI J. | BABAEIAN N.A. | RAHIMIAN H.A. | Issue Writer Certificate

Keywords

DAS-ELISAQ3

NCM-ELISAQ3

DIAGNOSTICSQ3

VIRUS FREE GERMPLASMQ3

POTATO VIRUSESQ3

Abstract

The simple, rapid and highly sensitive diagnosis of plant viruses using minimum plant material is a key element of virus free nuclear seed production in potato. Here we report a comparison of the efficiency of double antibody sandwich ELISA(DAS-ELISA) and nitrocellulose membrane ELISA (NCM-ELISA) methods for diagnosis of single and multiple infections of four important POTATO VIRUSES (PLRV, PVY, PVA and PVS) in samples suspected of being virus infected. Out of 150 samples tested by DAS-ELISA method, PLRV, PVY, PVA and PVS were detected in 14, 56, 61 and 38 samples, respectively, while with NCM-ELISA these numbers were 8, 49, 59 and 33 respectively. In general 46% of infected plants had only one virus and 54% showed multiple infection from which 88% were infected with two viruses. Number of plants tested positive in the leaf tissue using DAS-ELISA was higher than that by NCM-ELISA. DAS-ELISA required 0.2 gram of leaf tissue, while NCMELISA required less than 0.1 garm of tissue .Therefore NCM-ELISA test was more suitable for virus diagnosis of small plantlets in the tissue culture and DAS-ELISA technique was suitable for diagnosis of virus infection in the greenhouse and field grown plants.

Cites

No record.

References

No record.

Cite

APA: Copy

EMAMI MEYBODI, D., MOZAFARI, J., BABAEIAN, N.A., & RAHIMIAN, H.A.. (2007). EFFICIENCY OF SEROLOGICAL TECHNIQUES FOR DETECTING SINGLE AND MULTIPLE INFECTIONS OF MAJOR POTATO VIRUSES IN THE SEED PRODUCTION SYSTEM. IRANIAN JOURNAL OF PLANT PATHOLOGY, 43(3 (171)), 338-346. SID. https://sid.ir/paper/48353/en

Vancouver: Copy

EMAMI MEYBODI D., MOZAFARI J., BABAEIAN N.A., RAHIMIAN H.A.. EFFICIENCY OF SEROLOGICAL TECHNIQUES FOR DETECTING SINGLE AND MULTIPLE INFECTIONS OF MAJOR POTATO VIRUSES IN THE SEED PRODUCTION SYSTEM. IRANIAN JOURNAL OF PLANT PATHOLOGY[Internet]. 2007;43(3 (171)):338-346. Available from: https://sid.ir/paper/48353/en

IEEE: Copy

D. EMAMI MEYBODI, J. MOZAFARI, N.A. BABAEIAN, and H.A. RAHIMIAN, “EFFICIENCY OF SEROLOGICAL TECHNIQUES FOR DETECTING SINGLE AND MULTIPLE INFECTIONS OF MAJOR POTATO VIRUSES IN THE SEED PRODUCTION SYSTEM,” IRANIAN JOURNAL OF PLANT PATHOLOGY, vol. 43, no. 3 (171), pp. 338–346, 2007, [Online]. Available: https://sid.ir/paper/48353/en

Related Journal Papers