A CONDUCTOMETRIC UREA BIOSENSOR BY DIRECT 
IMMOBILIZATION OF UREASE ON PT ELECTRODE

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GHOURCHIAN H.; MOULAIE RAD A.; ELYASVANDI H.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Chemistry and Chemical Engineering Year:2004 | Volume:23 | Issue:2 Page(s): 55-63

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Information Journal Paper

Title

A CONDUCTOMETRIC UREA BIOSENSOR BY DIRECT IMMOBILIZATION OF UREASE ON PT ELECTRODE

Author(s)

GHOURCHIAN H. | MOULAIE RAD A. | ELYASVANDI H. | Issue Writer Certificate

Keywords

UREA DETERMINATION

CONDUCTOMETRIC BIOSENSOR

UREASE IMMOBILIZATION

ELECTRO-POLYMERIZATION

DIFFERENTIAL CONDUCTOMETRIC TRANSDUCER

UREASE COVALENT BINDING

Abstract

Urease was immobilized on platinum electrode both by chemical binding and electropolymerization.The conductometric urea biosensor thus prepared showed a detection limit of 4.9×10-5 M and linear dynamic range from 4.9×10-5 to 5.8×10-3 M for urea concentration when the enzyme is covalently immobilized on Pt electrodes. Conductometric transducers respond to the changes in ionic strength thereby leading to uncontrolled inaccuracies. Such interferences were effectively suppressed by here the use of differential sensor pairs. It was shown that measurements in diluted fluids are possible with the use of a reference sensor having no immobilized enzyme. Specifically, the sensor constructed by covalent binding provided more reproducible responses than that prepared by electropolymerization. Namely the former preserved 80% of its initial activity over a period of 25 days. In order to provide more reproducibility, a method was developed for regeneration of the sensor surface so that, the renewed sensor would responded to urea almost as new.

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APA: Copy

GHOURCHIAN, H., MOULAIE RAD, A., & ELYASVANDI, H.. (2004). A CONDUCTOMETRIC UREA BIOSENSOR BY DIRECT IMMOBILIZATION OF UREASE ON PT ELECTRODE . IRANIAN JOURNAL OF CHEMISTRY AND CHEMICAL ENGINEERING (IJCCE), 23(2), 55-63. SID. https://sid.ir/paper/530493/en

Vancouver: Copy

GHOURCHIAN H., MOULAIE RAD A., ELYASVANDI H.. A CONDUCTOMETRIC UREA BIOSENSOR BY DIRECT IMMOBILIZATION OF UREASE ON PT ELECTRODE . IRANIAN JOURNAL OF CHEMISTRY AND CHEMICAL ENGINEERING (IJCCE)[Internet]. 2004;23(2):55-63. Available from: https://sid.ir/paper/530493/en

IEEE: Copy

H. GHOURCHIAN, A. MOULAIE RAD, and H. ELYASVANDI, “A CONDUCTOMETRIC UREA BIOSENSOR BY DIRECT IMMOBILIZATION OF UREASE ON PT ELECTRODE ,” IRANIAN JOURNAL OF CHEMISTRY AND CHEMICAL ENGINEERING (IJCCE), vol. 23, no. 2, pp. 55–63, 2004, [Online]. Available: https://sid.ir/paper/530493/en

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