PROLIFERATE RESONSE TO PURIFIED BORDETELLA PERTUSSIS TOXIN ON MURINE SPLEEN LYMPHOCYTES

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POURAHMADI A.; ESMAEILI F.; ZAVARAN HOSSEINI A.; REZAEI A.; SALEHI E.; AKHAVIZADEGAN M.A.; MIRJALILI A.

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Journal: ARCHIVES OF RAZI INSTITUTE Year:2004 | Volume:- | Issue:58 Page(s): 63-71

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Title

PROLIFERATE RESONSE TO PURIFIED BORDETELLA PERTUSSIS TOXIN ON MURINE SPLEEN LYMPHOCYTES

Author(s)

Keywords

PERTUSSIS TOXIN

PURIFICATION

LYMPHOCYTE PROLIFERATION

SPLEEN CELL

Abstract

A PURIFICATION procedure of PERTUSSIS TOXIN (PT) from submerged culture of Bordetella pertussis (B.pertussis) strain 134 using adsorption and affinity chromatography was discussed. The yield of the resulting PT was approximately 37.5mg/l of concentrated culture supernatant. The polypeptide pattern of the purified PT was investigated by SOS-PAGE and showed five bands between 11 to 26 KDa using low molecular weight marker. Since PT is the maincomponent of acellular pertussis vaccine,obtaininga good yield of it would be essential for production of the new and safer vaccine generation. The other objective of this study was to determine the effects of PT on murine lymphocytes using MTT test. The effect of various doses of prepared PT on murine lymphocytes showed that the amount of 0.5µg/0.1m1 had the highest proliferation. Furthermore comparison between the resulting PT and phytohemagglutinin showed much higher effect of PT on murine SPLEEN CELLs. These results indicate that B.pertussis strain 134 is a suitable strain for induction of PT in order to use it for development of acellular vaccine in Iran and also for in vitro studies on proliferation of murine lymphocytes.

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APA: Copy

POURAHMADI, A., ESMAEILI, F., ZAVARAN HOSSEINI, A., REZAEI, A., SALEHI, E., AKHAVIZADEGAN, M.A., & MIRJALILI, A.. (2004). PROLIFERATE RESONSE TO PURIFIED BORDETELLA PERTUSSIS TOXIN ON MURINE SPLEEN LYMPHOCYTES. ARCHIVES OF RAZI INSTITUTE, -(58), 63-71. SID. https://sid.ir/paper/530635/en

Vancouver: Copy

POURAHMADI A., ESMAEILI F., ZAVARAN HOSSEINI A., REZAEI A., SALEHI E., AKHAVIZADEGAN M.A., MIRJALILI A.. PROLIFERATE RESONSE TO PURIFIED BORDETELLA PERTUSSIS TOXIN ON MURINE SPLEEN LYMPHOCYTES. ARCHIVES OF RAZI INSTITUTE[Internet]. 2004;-(58):63-71. Available from: https://sid.ir/paper/530635/en

IEEE: Copy

A. POURAHMADI, F. ESMAEILI, A. ZAVARAN HOSSEINI, A. REZAEI, E. SALEHI, M.A. AKHAVIZADEGAN, and A. MIRJALILI, “PROLIFERATE RESONSE TO PURIFIED BORDETELLA PERTUSSIS TOXIN ON MURINE SPLEEN LYMPHOCYTES,” ARCHIVES OF RAZI INSTITUTE, vol. -, no. 58, pp. 63–71, 2004, [Online]. Available: https://sid.ir/paper/530635/en

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