مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

493
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

391
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

1

Information Journal Paper

Title

USING L-ARABINOSE FOR PRODUCTION OF HUMAN GROWTH HORMONE IN ESCHERICHIA COLI, STUDYING THE PROCESSING OF GIII::HGH PRECURSOR

Pages

  250-260

Keywords

RECOMBINANT HUMAN GROWTH HORMONE (RHGH) 

Abstract

 Periplasmic expression of human growth hormone (hGH) in an ARABINOSE-regulated Escherichia coli system was studied, using two forms of gIII signal sequence differing from each other at position 17 (carrying either His17 or Arg17 at position -2 in the hGH precursor). The expression of hGH by the two recombinant plasmids was studied in the Top10 strain of E. coli. Results obtained from the expression analysis showed that the hGH expression in both of the recombinant bacteria are tightly regulated with ARABINOSE. An optimal expression was found to occur in the medium containing 1.33 mM L-arabinose at 37°C. However, PERIPLASMIC EXPRESSION of hGH occurs when the native signal sequence (His17-gIII) is applied. In the case of the bacteria carrying plasmid with the Arg17-gIII signal peptide, although the expression level was high, no mature protein could be detected under conditions tested. These data suggest for a probable effect of the -2 position in processing of the preprotein (gIII::hGH). Optimized growth and inducing conditions of the selected clone were investigated and application of a suitable SIGNAL PEPTIDE CLEAVAGE SITE for more efficient periplasmic production of hGH is discussed. The two recombinant plasmids presented in this work, have provided tools to study an aspect of amino acid sequence in the cleavage region on the processing and secretion of hGH.

Cites

References

  • No record.

    • Cite

    APA: Copy

    GHASEMI, F., ZOMORODIPOUR, A.R., SHOJAI, SH., ATAEI, F., KHODABANDEH, M., & SANATI, M.H.. (2004). USING L-ARABINOSE FOR PRODUCTION OF HUMAN GROWTH HORMONE IN ESCHERICHIA COLI, STUDYING THE PROCESSING OF GIII::HGH PRECURSOR . IRANIAN JOURNAL OF BIOTECHNOLOGY, 2(4), 250-260. SID. https://sid.ir/paper/530935/en

    Vancouver: Copy

    GHASEMI F., ZOMORODIPOUR A.R., SHOJAI SH., ATAEI F., KHODABANDEH M., SANATI M.H.. USING L-ARABINOSE FOR PRODUCTION OF HUMAN GROWTH HORMONE IN ESCHERICHIA COLI, STUDYING THE PROCESSING OF GIII::HGH PRECURSOR . IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2004;2(4):250-260. Available from: https://sid.ir/paper/530935/en

    IEEE: Copy

    F. GHASEMI, A.R. ZOMORODIPOUR, SH. SHOJAI, F. ATAEI, M. KHODABANDEH, and M.H. SANATI, “USING L-ARABINOSE FOR PRODUCTION OF HUMAN GROWTH HORMONE IN ESCHERICHIA COLI, STUDYING THE PROCESSING OF GIII::HGH PRECURSOR ,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 2, no. 4, pp. 250–260, 2004, [Online]. Available: https://sid.ir/paper/530935/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops





    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button