GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM STREPTOMYCES AUREOFACIENS: LIGAND-INDUCED CONFORMATIONAL CHANG

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HAGHIGHI B.; AGHATABAR A.M.; SHAHSAVARI GH.

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Journal Paper

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Journal: Iranian Journal of Science Year:2005 | Volume:29 | Issue:A1 Page(s): 71-78

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Title

GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM STREPTOMYCES AUREOFACIENS: LIGAND-INDUCED CONFORMATIONAL CHANG

Author(s)

HAGHIGHI B. | AGHATABAR A.M. | SHAHSAVARI GH. | Issue Writer Certificate

Keywords

GLUCOSE 6-PHOSPHATE DEHYDROGENASE

STREPTOMYCES AUREOFACIENS

Abstract

Some kinetic properties of NAD+- and NADP+- dependent GLUCOSE 6-PHOSPHATE DEHYDROGENASE (G6PD) purified from STREPTOMYCES AUREOFACIENS were studied. Both NADH and NADPH inhibited the enzyme competitively and noncompetitively, with respect to the corresponding coenzymes and glucose 6-phosphate, respectively. ATP inhibited the NAD+ - linked reaction but not that of the NADP+- linked activity. The inhibition was competitive with respect to NAD+ and noncompetitive with respect to glucose 6-phosphate. Km values were 0.14 mM for NAD+ and 0.075 mM for NADP+. Similar Km values (0.75-0.79 mM) were obtaind for glucose 6-phosphate using either NAD+ or NADP+ as a coenzyme. The optimum pH was 6.6 for NAD+- and 7.4 for NADP+- dependent activity. Maximum protein fluorescence was increased by NAD+ (49%) and NADP+ (8%). Among bivalent cations studied, Cu2+ decreased NAD+- linked activity (40%), but increased the NADP+- linked reaction (10%). Ni2+ did not affect NAD+- linked, but stimulated NADP+- linked activity. Other cations such as Zn2+ and Mn2+ also differently affected the two reactions. The data suggested that binding of NAD+ and NADP+ produces a different conformational change in S. aureofaciens G6PD or an isomerisation process regulates coenzyme utilization.

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APA: Copy

HAGHIGHI, B., AGHATABAR, A.M., & SHAHSAVARI, GH.. (2005). GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM STREPTOMYCES AUREOFACIENS: LIGAND-INDUCED CONFORMATIONAL CHANG. IRANIAN JOURNAL OF SCIENCE AND TECHNOLOGY TRANSACTION A- SCIENCE, 29(A1), 71-78. SID. https://sid.ir/paper/531901/en

Vancouver: Copy

HAGHIGHI B., AGHATABAR A.M., SHAHSAVARI GH.. GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM STREPTOMYCES AUREOFACIENS: LIGAND-INDUCED CONFORMATIONAL CHANG. IRANIAN JOURNAL OF SCIENCE AND TECHNOLOGY TRANSACTION A- SCIENCE[Internet]. 2005;29(A1):71-78. Available from: https://sid.ir/paper/531901/en

IEEE: Copy

B. HAGHIGHI, A.M. AGHATABAR, and GH. SHAHSAVARI, “GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM STREPTOMYCES AUREOFACIENS: LIGAND-INDUCED CONFORMATIONAL CHANG,” IRANIAN JOURNAL OF SCIENCE AND TECHNOLOGY TRANSACTION A- SCIENCE, vol. 29, no. A1, pp. 71–78, 2005, [Online]. Available: https://sid.ir/paper/531901/en

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