PURIFICATION OF LARGE QUANTITIES OF BIOLOGICALLY ACTIVE RECOMBINANT HUMAN GROWTH HORMONE

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KHODABANDEH M.; YAKHCHALI B.; RAHIMI M.; VASELI N.; MOGHADDASI JAHROMI Z.; ZOMORODIPOUR A.R.; DIZAJI A.A.KH.; ANSARI
MAJD S.; SANATI M.H.

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Journal Paper

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Journal: Iranian Journal of Biotechnology Year:2003 | Volume:1 | Issue:4 Page(s): 207-212

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Title

PURIFICATION OF LARGE QUANTITIES OF BIOLOGICALLY ACTIVE RECOMBINANT HUMAN GROWTH HORMONE

Author(s)

Keywords

RECOMBINANT PROTEIN

HGH

E. COLI

PURIFICATION.

Abstract

Production and PURIFICATION.of human growth hormone using a simple method was studied in two recombinant Escherichia coli, D7-5 and C27-2 strains. The r-hGH was expressed in the form of inclusion body in a batch fermentation process and purified to 99% purity using a procedure based on acid precipitation of the host derived proteins and other impurities. The effect of the pH and host strain on PURIFICATION.of the r-hGH and efficiency of the procedure were evaluated. It was found that the optimum pH for precipitation of the host derived proteins was 4.9. The procedure was suitable for r-hGH PURIFICATION.from D7-5 stain but not from the other strain C27-2. The purity of > 99% and recovery of about 40% were obtained as shown by SDS-PAGE and Western blot analysis. The purified r-hGH was biologically active as judged by receptor assay with very low endotoxin content which could be suitable for therapeutic applications. This simple and cost effective production process could be useful for large scale production of recombinant HGH from specific strains.

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APA: Copy

KHODABANDEH, M., YAKHCHALI, B., RAHIMI, M., VASELI, N., MOGHADDASI JAHROMI, Z., ZOMORODIPOUR, A.R., DIZAJI, A.A.KH., ANSARI MAJD, S., & SANATI, M.H.. (2003). PURIFICATION OF LARGE QUANTITIES OF BIOLOGICALLY ACTIVE RECOMBINANT HUMAN GROWTH HORMONE. IRANIAN JOURNAL OF BIOTECHNOLOGY, 1(4), 207-212. SID. https://sid.ir/paper/531954/en

Vancouver: Copy

KHODABANDEH M., YAKHCHALI B., RAHIMI M., VASELI N., MOGHADDASI JAHROMI Z., ZOMORODIPOUR A.R., DIZAJI A.A.KH., ANSARI MAJD S., SANATI M.H.. PURIFICATION OF LARGE QUANTITIES OF BIOLOGICALLY ACTIVE RECOMBINANT HUMAN GROWTH HORMONE. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2003;1(4):207-212. Available from: https://sid.ir/paper/531954/en

IEEE: Copy

M. KHODABANDEH, B. YAKHCHALI, M. RAHIMI, N. VASELI, Z. MOGHADDASI JAHROMI, A.R. ZOMORODIPOUR, A.A.KH. DIZAJI, S. ANSARI MAJD, and M.H. SANATI, “PURIFICATION OF LARGE QUANTITIES OF BIOLOGICALLY ACTIVE RECOMBINANT HUMAN GROWTH HORMONE,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 1, no. 4, pp. 207–212, 2003, [Online]. Available: https://sid.ir/paper/531954/en

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