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Information Journal Paper

Title

PURIFICATION AND CHARACTERIZATION OF TWO ACID PHOSPHATASES FROM GERMINATING PEANUT (ARACHIS HYPOGAEA) SEED

Pages

  26-35

Abstract

 The maximum acid phosphatasic activity was detected in PEANUT seed at the 5th day of GERMINATION. At least, two ACID PHOSPHATASEs were purified by successive chromatography separations on DEAE-Sepharose CL-6B, CM-Sepharose CL-6B, Sephacryl S-100 HR, and Phenyl-Sepharose HP to apparent homogeneity from five days old COTYLEDON of PEANUT after GERMINATION. These isoenzymes, designated PEANUT COTYLEDON ACID PHOSPHATASE 1 and 2 (PCAP 1 and PCAP 2), had native molecular weights of approximately 27.5 and 24 kDa by gel permeation, respectively. SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) of PCAP 1 and PCAP 2 resolved a single protein band (each) that migrated to approximately 27 and 29 kDa, respectively. Thus, these ACID PHOSPHATASEs likely function as a monomer. The two isoenzymes had a similar optimum temperature (55°C), two closely optima pH (5.6 and 5.0), and appeared to be stable in the presence of some detergents such as Triton X-100, Nonidet P-40, Taurocholic acid sodium salt, Polyoxyethylene-9-lauryl ether as well as Mg2+, Sr2+, Fe3+ and Ba2+. Substrate specificity indicated that PCAP 1 and PCAP 2 hydrolyzed a broad range of phosphorylated substrates. However, natural substrates such as ADP, ATP and phenylphosphate had the highest rate of hydrolysis for the two isoenzymes.

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    Cite

    APA: Copy

    TIA GONNETY, J., NIAMKE, S., MEUWIAH FAULET, B., N GUESSAN KOUADIO, E.J.P., & KOUAME, L.P.. (2006). PURIFICATION AND CHARACTERIZATION OF TWO ACID PHOSPHATASES FROM GERMINATING PEANUT (ARACHIS HYPOGAEA) SEED. IRANIAN JOURNAL OF BIOTECHNOLOGY, 4(1), 26-35. SID. https://sid.ir/paper/536890/en

    Vancouver: Copy

    TIA GONNETY J., NIAMKE S., MEUWIAH FAULET B., N GUESSAN KOUADIO E.J.P., KOUAME L.P.. PURIFICATION AND CHARACTERIZATION OF TWO ACID PHOSPHATASES FROM GERMINATING PEANUT (ARACHIS HYPOGAEA) SEED. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2006;4(1):26-35. Available from: https://sid.ir/paper/536890/en

    IEEE: Copy

    J. TIA GONNETY, S. NIAMKE, B. MEUWIAH FAULET, E.J.P. N GUESSAN KOUADIO, and L.P. KOUAME, “PURIFICATION AND CHARACTERIZATION OF TWO ACID PHOSPHATASES FROM GERMINATING PEANUT (ARACHIS HYPOGAEA) SEED,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 4, no. 1, pp. 26–35, 2006, [Online]. Available: https://sid.ir/paper/536890/en

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