DETECTION OF BOVINE VIRAL DIARRHEA VIRUS USING A NESTED RT-PCR ASSAY IN BULK MILK SAMPLES OF DAIRY CATTLE HERDS IN SUBURB OF MASHHAD-IRAN

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TALEBKHAN GAROUSI M.; BASAMI M.R.; AFSHARI S.E.

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Journal Paper

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Journal: Iranian Journal of Biotechnology Year:2007 | Volume:5 | Issue:1 Page(s): 52-55

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Information Journal Paper

Title

DETECTION OF BOVINE VIRAL DIARRHEA VIRUS USING A NESTED RT-PCR ASSAY IN BULK MILK SAMPLES OF DAIRY CATTLE HERDS IN SUBURB OF MASHHAD-IRAN

Author(s)

TALEBKHAN GAROUSI M. | BASAMI M.R. | AFSHARI S.E. | Issue Writer Certificate

Keywords

BVD VIRUS

NESTED RT- PCR

BULK MILK

CATTLE

Abstract

Bovine viral diarrhoea virus (BVDV) is an important pathogen of dairy CATTLE. In this study, BULK MILK samples representing a total of 4105 milking cows, from 18 dairy CATTLE herds in the suburb of Mashhad- Iran, were tested for presence of BVDV by the use of a nested reverse transcription polymerase chain reaction (Nested RT- PCR) assay. Non of the cows in the herds had been vaccinated against BVDV. RNA was extracted from somatic cell pellets of BULK MILK tank samples. Oligonucleotide primers were selected based on the 5´untranslated region of the BVD VIRUS genome. BVD VIRUS was detected in 2 (11.1%) out of 18 samples, representing 742 lactating cows. These results indicate that nested RT-PCR analysis of BULK MILK samples may provide a rapid and sensitive screening method for the detection of BVDV infections in non-vaccinated dairy CATTLE herds.

Cites

SEROLOGICAL STUDY OF BOVINE VIRAL RESPIRATORY DISEASES IN DAIRY HERDS IN KERMAN PROVINCE, IRAN

References

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APA: Copy

TALEBKHAN GAROUSI, M., BASAMI, M.R., & AFSHARI, S.E.. (2007). DETECTION OF BOVINE VIRAL DIARRHEA VIRUS USING A NESTED RT-PCR ASSAY IN BULK MILK SAMPLES OF DAIRY CATTLE HERDS IN SUBURB OF MASHHAD-IRAN. IRANIAN JOURNAL OF BIOTECHNOLOGY, 5(1), 52-55. SID. https://sid.ir/paper/553167/en

Vancouver: Copy

TALEBKHAN GAROUSI M., BASAMI M.R., AFSHARI S.E.. DETECTION OF BOVINE VIRAL DIARRHEA VIRUS USING A NESTED RT-PCR ASSAY IN BULK MILK SAMPLES OF DAIRY CATTLE HERDS IN SUBURB OF MASHHAD-IRAN. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2007;5(1):52-55. Available from: https://sid.ir/paper/553167/en

IEEE: Copy

M. TALEBKHAN GAROUSI, M.R. BASAMI, and S.E. AFSHARI, “DETECTION OF BOVINE VIRAL DIARRHEA VIRUS USING A NESTED RT-PCR ASSAY IN BULK MILK SAMPLES OF DAIRY CATTLE HERDS IN SUBURB OF MASHHAD-IRAN,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 5, no. 1, pp. 52–55, 2007, [Online]. Available: https://sid.ir/paper/553167/en

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