ISOLATION AND MOLECULAR DETERMINATION OF PHENOL DEGRADING BACTERIA

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HASAN SHAHIAN M.; GOLBANG N.; EMTIAZI G.

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Journal: RESEARCH JOURNAL OF UNIVERSITY OF ISFAHAN "SCIENCE" Year:2007 | Volume:28 | Issue:2 Page(s): 1-8

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Information Journal Paper

Title

ISOLATION AND MOLECULAR DETERMINATION OF PHENOL DEGRADING BACTERIA

Author(s)

HASAN SHAHIAN M. | GOLBANG N. | EMTIAZI G. | Issue Writer Certificate

Keywords

PCRQ4

PHENOLQ4

PHENOL HYDROXYLASEQ4

MPN SOIL AND PHENOL-DEGRADING BACTERIAQ4

Abstract

To determine PHENOL contaminated soils, forty five PHENOL-degrading bacteria were isolated from soil and wastewater samples in PHENOL agar after several subcultures in PHENOL broth. All isolates utilized 0.2 g/l PHENOL and were identified as Pseudomonas sp. PCR amplification of LmPH and dmpN genes showed that all 45 isolated strains contained LmPH genes and 12 strains were Pseudomonas putida. The LmPH gene were detected in contaminated and uncontaminated soil, although microtiter MPN methods showed that the quantity of PHENOL-degrading bacteria in contaminated soil was more than in uncontaminated soil. The soil contamination might be identified by PCR of some genes; however the presence of PHENOL in all studied soil showed positive reaction in contaminated and uncontaminated natural soil. The results indicated that the PHENOL degrader bacteria are distributed in all soils.

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APA: Copy

HASAN SHAHIAN, M., GOLBANG, N., & EMTIAZI, G.. (2007). ISOLATION AND MOLECULAR DETERMINATION OF PHENOL DEGRADING BACTERIA. RESEARCH JOURNAL OF UNIVERSITY OF ISFAHAN "SCIENCE", 28(2), 1-8. SID. https://sid.ir/paper/55799/en

Vancouver: Copy

HASAN SHAHIAN M., GOLBANG N., EMTIAZI G.. ISOLATION AND MOLECULAR DETERMINATION OF PHENOL DEGRADING BACTERIA. RESEARCH JOURNAL OF UNIVERSITY OF ISFAHAN "SCIENCE"[Internet]. 2007;28(2):1-8. Available from: https://sid.ir/paper/55799/en

IEEE: Copy

M. HASAN SHAHIAN, N. GOLBANG, and G. EMTIAZI, “ISOLATION AND MOLECULAR DETERMINATION OF PHENOL DEGRADING BACTERIA,” RESEARCH JOURNAL OF UNIVERSITY OF ISFAHAN "SCIENCE", vol. 28, no. 2, pp. 1–8, 2007, [Online]. Available: https://sid.ir/paper/55799/en

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