IMMUNIZATION OF ARABIAN SHEEP WITH WHOLE GUT HOMOGENATE OF HAEMONCHUS CONTORTUS

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NAYEBZADEH H.; SEYFIABAD SHAPOURI M.; HOGHOUGHIRAD N.; MASOURI B.

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Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF VETERINARY RESEARCH Year:2008 | Volume:9 | Issue:4 (25) Page(s): 360-364

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Information Journal Paper

Title

IMMUNIZATION OF ARABIAN SHEEP WITH WHOLE GUT HOMOGENATE OF HAEMONCHUS CONTORTUS

Author(s)

NAYEBZADEH H. | SEYFIABAD SHAPOURI M. | HOGHOUGHIRAD N. | MASOURI B. | Issue Writer Certificate

Keywords

HAEMONCHUS CONTORTUS

VACCINATION

WHOLE GUT HOMOGENATE

ARABIAN SHEEP

Abstract

Ten female lambs of 7-month-old were divided into two equal groups and raised under a helminth-free condition. Animals in group 1 were immunized two times by whole gut homogenate (WGH) of Haemonchus contortus emulsified in Freund’s adjuvant. In group 2 (control), animals were injected by phosphate buffered saline emulsified in the same adjuvant. Animals were challenged by 10000 third-stage larvae (L3) of Haemonchus contortus on day 33 after the first immunization and then humanely killed on day 42. Animals were tested for serum antibody and eggs per gram of faeces (EPG) throughout the study and nematodes in their abomasom were counted after necropsy. The results indicated that animals immunized with WGH showed a higher level of serum antibodies. A significant difference was observed in mean optical density of sera in ELISA between the two groups (P<0.05) and a 77 and 78% reduction in EPG and nematode counts at necropsy, respectively (P<0.05).

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APA: Copy

NAYEBZADEH, H., SEYFIABAD SHAPOURI, M., HOGHOUGHIRAD, N., & MASOURI, B.. (2008). IMMUNIZATION OF ARABIAN SHEEP WITH WHOLE GUT HOMOGENATE OF HAEMONCHUS CONTORTUS. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), 9(4 (25)), 360-364. SID. https://sid.ir/paper/563455/en

Vancouver: Copy

NAYEBZADEH H., SEYFIABAD SHAPOURI M., HOGHOUGHIRAD N., MASOURI B.. IMMUNIZATION OF ARABIAN SHEEP WITH WHOLE GUT HOMOGENATE OF HAEMONCHUS CONTORTUS. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR)[Internet]. 2008;9(4 (25)):360-364. Available from: https://sid.ir/paper/563455/en

IEEE: Copy

H. NAYEBZADEH, M. SEYFIABAD SHAPOURI, N. HOGHOUGHIRAD, and B. MASOURI, “IMMUNIZATION OF ARABIAN SHEEP WITH WHOLE GUT HOMOGENATE OF HAEMONCHUS CONTORTUS,” IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), vol. 9, no. 4 (25), pp. 360–364, 2008, [Online]. Available: https://sid.ir/paper/563455/en

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