SIMPLE AND EFFECTIVE METHOD FOR GENOMIC DNA EXTRACTION FROM PLANT AND FUNGUS FOR PCR-BASED ANALYSES

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NOUROUZI PARVIZ

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Journal: Journal of Sugar Beet Year:2004 | Volume:19 | Issue:2 Page(s): 175-177

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Title

SIMPLE AND EFFECTIVE METHOD FOR GENOMIC DNA EXTRACTION FROM PLANT AND FUNGUS FOR PCR-BASED ANALYSES

Author(s)

NOUROUZI PARVIZ | Issue Writer Certificate

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Abstract

There are a number of methods (protocols) used in different laboratories for extraction of genomic DNA from plant and fungus sources, dependening on the quantity and quality of the required DNA. However, the basic principals of DNA extraction procedures are the same in most of them (2, 4-6). Regarding researches based on molecular marker conducted at the biotechnology laboratory of Sugar Beet Seed Institute, There seems to be a simple, low cost and efficient method needed for genomic DNA extraction from plant or fungus materials. The extracted DNAs are prerequisite to accomplish PCR-based molecular analysis. Therefore, some DNA extraction protocols were compared (1-6) and a simple and repeatable method useful for plant and fungus materials was obtained. Finally, the protocol was applied and the extracted DNAs from two sources of fungus and plant were considered as template DNAs in specific PCR and RAPD analysis after quantification and qualification. DNA banding patterns showed that the DNA extraction protocol was efficient for both sources (Fig. 1). This DNA extraction method takes less than three hours and compared to the most commonly used methods in molecular biology laboratories it has been proved to have lower cost.

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APA: Copy

NOUROUZI, PARVIZ. (2004). SIMPLE AND EFFECTIVE METHOD FOR GENOMIC DNA EXTRACTION FROM PLANT AND FUNGUS FOR PCR-BASED ANALYSES . JOURNAL OF SUGAR BEET, 19(2), 175-177. SID. https://sid.ir/paper/57556/en

Vancouver: Copy

NOUROUZI PARVIZ. SIMPLE AND EFFECTIVE METHOD FOR GENOMIC DNA EXTRACTION FROM PLANT AND FUNGUS FOR PCR-BASED ANALYSES . JOURNAL OF SUGAR BEET[Internet]. 2004;19(2):175-177. Available from: https://sid.ir/paper/57556/en

IEEE: Copy

PARVIZ NOUROUZI, “SIMPLE AND EFFECTIVE METHOD FOR GENOMIC DNA EXTRACTION FROM PLANT AND FUNGUS FOR PCR-BASED ANALYSES ,” JOURNAL OF SUGAR BEET, vol. 19, no. 2, pp. 175–177, 2004, [Online]. Available: https://sid.ir/paper/57556/en

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