مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

PURIFICATION AND PARTIAL CHARACTERIZATION OF A THROMBIN-LIKE ENZYME (AH144) FROM VENOM OF IRANIAN SNAKE AGKISTRODON HALYS

Pages

  103-109

Abstract

 The snake venom´s THROMBIN-LIKE ENZYMEscomprise a number of serine proteases, which are functionally and structurally related to thrombin. PURIFICATION and partial characterization of a THROMBIN-LIKE ENZYME from the venom of the Iranian snake, AGKISTRODON HALYS, was the aim of this study. PURIFICATION was carried out by a combination of variety of chromatographic methods that included: gel filtration on Sephadex G-50, ion-exchange chromatography on DEAE-Sepharose and HPLC with a C18 column. A trial for the PURIFICATION of protease resulted in an enzyme with specific activity of 721.2 (mmol/min/mg), which was purified by 72.1 fold. The purified THROMBIN-LIKE ENZYME designated AH144 was found to have a molecular weight of approximately 30.5 kDa. This THROMBIN-LIKE ENZYME had the highest activity at 37oC and pH 7.5. Enzyme activity increased as its concentration increased, and the purified enzyme did not have any effect on casein. AH144 demonstrated clotting and proteolytic activities in the presence of the human plasma and the synthetic substrate (BApNA), respectively. Data emphasized the possibility of AH144 for quantitative determination of fibrinogen.

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    APA: Copy

    GHORBANPOUR, MOHAMMAD, ZOKAEE ASHTIANI, FARZIN, ZARE MIRAKABADI, ABBAS, & ZOLFAGHARIAN, HOSEIN. (2012). PURIFICATION AND PARTIAL CHARACTERIZATION OF A THROMBIN-LIKE ENZYME (AH144) FROM VENOM OF IRANIAN SNAKE AGKISTRODON HALYS. IRANIAN JOURNAL OF CHEMISTRY AND CHEMICAL ENGINEERING (IJCCE), 31(2 (62)), 103-109. SID. https://sid.ir/paper/582602/en

    Vancouver: Copy

    GHORBANPOUR MOHAMMAD, ZOKAEE ASHTIANI FARZIN, ZARE MIRAKABADI ABBAS, ZOLFAGHARIAN HOSEIN. PURIFICATION AND PARTIAL CHARACTERIZATION OF A THROMBIN-LIKE ENZYME (AH144) FROM VENOM OF IRANIAN SNAKE AGKISTRODON HALYS. IRANIAN JOURNAL OF CHEMISTRY AND CHEMICAL ENGINEERING (IJCCE)[Internet]. 2012;31(2 (62)):103-109. Available from: https://sid.ir/paper/582602/en

    IEEE: Copy

    MOHAMMAD GHORBANPOUR, FARZIN ZOKAEE ASHTIANI, ABBAS ZARE MIRAKABADI, and HOSEIN ZOLFAGHARIAN, “PURIFICATION AND PARTIAL CHARACTERIZATION OF A THROMBIN-LIKE ENZYME (AH144) FROM VENOM OF IRANIAN SNAKE AGKISTRODON HALYS,” IRANIAN JOURNAL OF CHEMISTRY AND CHEMICAL ENGINEERING (IJCCE), vol. 31, no. 2 (62), pp. 103–109, 2012, [Online]. Available: https://sid.ir/paper/582602/en

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