MICROPROPAGATION OF ALTERNANTHERA SESSILIS (L.) USING SHOOT TIP AND NODAL SEGMENTS

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GNANARAJ WESELY EDWARD; ALIAS ANTONISAMY JOHNSON MARIMUTHU; SUBRAMANIAN KAVITHA MARAPPAMPALAYAM; NALLYAN SELVAN

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Journal Paper

Paper Information

Journal: Iranian Journal of Biotechnology Year:2011 | Volume:9 | Issue:3 Page(s): 206-212

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Title

MICROPROPAGATION OF ALTERNANTHERA SESSILIS (L.) USING SHOOT TIP AND NODAL SEGMENTS

Author(s)

GNANARAJ WESELY EDWARD | ALIAS ANTONISAMY JOHNSON MARIMUTHU | SUBRAMANIAN KAVITHA MARAPPAMPALAYAM | NALLYAN SELVAN | Issue Writer Certificate

Keywords

IN VITRO

CALLI

CLONAL

SHOOT TIP

NODAL SEGMENTS

Abstract

A rapid IN VITRO propagation system has been established from mature SHOOT TIP and NODAL SEGMENTS of a highly valuable medicinal plant Alternanthera sessilis (L.). The explants were cultured on Murashige and Skoog’s medium augmented with different concentrations and combinations of plant growth regulators for shoot bud initiation and multiplications. For SHOOT TIP, highest frequency of shoot proliferation (94.3±0.43) and maximum number per explants (23.4±0.38) was observed in Murashige and Skoog’s medium augmented with 2.0 mg/l of 6-Benzyl Amino Purine. For NODAL SEGMENTS, highest frequency of shoot proliferation (90.4±0.82) and maximum number (15.2±0.63) per node was observed in Murashige and Skoog’s medium augmented with 1.5 mg/l of 6-Benzyl Amino Purine.Maximum percentage of callus formation (Leaves- 92.4±0.61; Inter-nodal -88.9±0.83) was obtained on Murashige and Skoog’s basal medium supplemented with 3% and 2, 4-Dichlorophenoxy acetic acid 2.0 mg/l.Highest efficiency (97.4±1.36) of rooting and maximum number (6.3±0.42) of rootlet per shoot let was achieved on half strength Murashige and Skoog’s medium fortified with 3 mg/l of Indole-3-Butyric acid.Regenerated plants were successfully transferred to field (78%).

Cites

DIRECT MULTIPLE SHOOT REGENERATION FROM SHOOT TIP AND NODAL EXPLANTS OF SOLANUM NIGRUM L. A MEDICINAL HERB

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APA: Copy

GNANARAJ, WESELY EDWARD, ALIAS ANTONISAMY, JOHNSON MARIMUTHU, SUBRAMANIAN, KAVITHA MARAPPAMPALAYAM, & NALLYAN, SELVAN. (2011). MICROPROPAGATION OF ALTERNANTHERA SESSILIS (L.) USING SHOOT TIP AND NODAL SEGMENTS. IRANIAN JOURNAL OF BIOTECHNOLOGY, 9(3), 206-212. SID. https://sid.ir/paper/582762/en

Vancouver: Copy

GNANARAJ WESELY EDWARD, ALIAS ANTONISAMY JOHNSON MARIMUTHU, SUBRAMANIAN KAVITHA MARAPPAMPALAYAM, NALLYAN SELVAN. MICROPROPAGATION OF ALTERNANTHERA SESSILIS (L.) USING SHOOT TIP AND NODAL SEGMENTS. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2011;9(3):206-212. Available from: https://sid.ir/paper/582762/en

IEEE: Copy

WESELY EDWARD GNANARAJ, JOHNSON MARIMUTHU ALIAS ANTONISAMY, KAVITHA MARAPPAMPALAYAM SUBRAMANIAN, and SELVAN NALLYAN, “MICROPROPAGATION OF ALTERNANTHERA SESSILIS (L.) USING SHOOT TIP AND NODAL SEGMENTS,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 9, no. 3, pp. 206–212, 2011, [Online]. Available: https://sid.ir/paper/582762/en

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