PRODUCTION AND EVALUATION OF POLYCLONAL RABBIT ANTIHUMAN P53 ANTIBODY USING BACTERIALLY EXPRESSED GLUTATHIONE S-TRANSFERASE-P53 FUSION PROTEIN

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RASTI MOZHGAN; KHATOONI ZAHED; MOSTAFAVI POUR ZOHERH

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Journal Paper

Paper Information

Journal: Iranian Journal of Biotechnology Year:2012 | Volume:10 | Issue:4 Page(s): 240-248

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Information Journal Paper

Title

PRODUCTION AND EVALUATION OF POLYCLONAL RABBIT ANTIHUMAN P53 ANTIBODY USING BACTERIALLY EXPRESSED GLUTATHIONE S-TRANSFERASE-P53 FUSION PROTEIN

Author(s)

RASTI MOZHGAN | KHATOONI ZAHED | MOSTAFAVI POUR ZOHERH | Issue Writer Certificate

Keywords

GST-P53

TUMOR

IMMUNOASSAYS

ANTIP53 ANTIBODY

Abstract

p53is a key TUMOR suppressor gene that is targeted for inactivation during human TUMORigenesis. In this study, we produced and characterized polyclonal antihuman p53 antibody. The cDNA encoding the complete human p53 protein was cloned into pGEX-4T-1 and expressed in Escherichia coli as a fusion protein with Schistosoma japonicum glutathione S-transferase (GST). The rabbits were immunized with the purified p53 recombinant protein. The obtained antisera were purified to increase the specificity of recognition. The sensitivity and specificity of the produced antibody was analyzed by enzyme-linked immunosorbent, immunoblot, immunofluorescence and chromatin immunoprecipitation assays. Enzyme-linked immunosorbent assay showed that immunization with purified GST-P53 produced the high titer (1: 10000) polyclonal antibodies with high specificity. Anti-p53 antibody allowed the sensitive detection of native p53 protein in immunoblotting, immunofluorescence and chromatin immunoprecipitation assays. Our results showed that anti-GST-P53 antibody provides a good means for studying the p53 expression pattern and its binding ability to other proteins in TUMORs.

Cites

TP53 BINDING TO BRCA1 AND RAD51 IN MCF7 AND MDA-MB-468 BREAST CANCER CELL LINES IN VIVO AND IN VITRO

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APA: Copy

RASTI, MOZHGAN, KHATOONI, ZAHED, & MOSTAFAVI POUR, ZOHERH. (2012). PRODUCTION AND EVALUATION OF POLYCLONAL RABBIT ANTIHUMAN P53 ANTIBODY USING BACTERIALLY EXPRESSED GLUTATHIONE S-TRANSFERASE-P53 FUSION PROTEIN. IRANIAN JOURNAL OF BIOTECHNOLOGY, 10(4), 240-248. SID. https://sid.ir/paper/586397/en

Vancouver: Copy

RASTI MOZHGAN, KHATOONI ZAHED, MOSTAFAVI POUR ZOHERH. PRODUCTION AND EVALUATION OF POLYCLONAL RABBIT ANTIHUMAN P53 ANTIBODY USING BACTERIALLY EXPRESSED GLUTATHIONE S-TRANSFERASE-P53 FUSION PROTEIN. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2012;10(4):240-248. Available from: https://sid.ir/paper/586397/en

IEEE: Copy

MOZHGAN RASTI, ZAHED KHATOONI, and ZOHERH MOSTAFAVI POUR, “PRODUCTION AND EVALUATION OF POLYCLONAL RABBIT ANTIHUMAN P53 ANTIBODY USING BACTERIALLY EXPRESSED GLUTATHIONE S-TRANSFERASE-P53 FUSION PROTEIN,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 10, no. 4, pp. 240–248, 2012, [Online]. Available: https://sid.ir/paper/586397/en

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