PROTEOME ANALYSIS OF CRYPTOSPORIDIUM PARVUM AND C. HOMINIS USING TWO-DIMENTIONAL ELECTROPHORESIS, IMAGE ANALYSIS AND TANDEM MASS SPECTROMETRY

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ZONAED SIDDIKI AMAM

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Iranian Journal of Biotechnology Year:2012 | Volume:10 | Issue:3 Page(s): 198-207

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Title

PROTEOME ANALYSIS OF CRYPTOSPORIDIUM PARVUM AND C. HOMINIS USING TWO-DIMENTIONAL ELECTROPHORESIS, IMAGE ANALYSIS AND TANDEM MASS SPECTROMETRY

Author(s)

ZONAED SIDDIKI AMAM | Issue Writer Certificate

Keywords

COMPARATIVE PROTEOMICS

CRYPTOSPORIDIUM

BIOINFORMATICS

2 DE IMAGE ANALYSIS

MASS SPECTROMETRY

Abstract

Until recently, CRYPTOSPORIDIUM was thought to be a single species genus. Molecular studies now show that there are at least 10 valid species of this parasite.Among them, two morphologically identical species, C. hominisand C. parvum are the most pathogenic identified to date and share 97% of identical genomes.Post-genomic analyses is therefore necessary to explore further the genetic variations among them.During this study, a comparative proteomic approach was applied to analyze the differential expression of sporozoite proteins in bothC. hominis and C. parvum.Using 2-DE gels with different pH ranges (3-10 and 4-7) and automated three dimensional (3D) image analyses, a total of 20 protein spots were shown to be differentially expressed between the two species. MASS SPECTROMETRY analyses of these spots identified one hypothetical protein, however, identification of the remaining spots was unsuccessful. Further characterization of this hypothetical protein along with all differentially expressed proteins could provide crucial information in understanding the differential biology of CRYPTOSPORIDIUM spp.

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APA: Copy

ZONAED SIDDIKI, AMAM. (2012). PROTEOME ANALYSIS OF CRYPTOSPORIDIUM PARVUM AND C. HOMINIS USING TWO-DIMENTIONAL ELECTROPHORESIS, IMAGE ANALYSIS AND TANDEM MASS SPECTROMETRY. IRANIAN JOURNAL OF BIOTECHNOLOGY, 10(3), 198-207. SID. https://sid.ir/paper/604379/en

Vancouver: Copy

ZONAED SIDDIKI AMAM. PROTEOME ANALYSIS OF CRYPTOSPORIDIUM PARVUM AND C. HOMINIS USING TWO-DIMENTIONAL ELECTROPHORESIS, IMAGE ANALYSIS AND TANDEM MASS SPECTROMETRY. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2012;10(3):198-207. Available from: https://sid.ir/paper/604379/en

IEEE: Copy

AMAM ZONAED SIDDIKI, “PROTEOME ANALYSIS OF CRYPTOSPORIDIUM PARVUM AND C. HOMINIS USING TWO-DIMENTIONAL ELECTROPHORESIS, IMAGE ANALYSIS AND TANDEM MASS SPECTROMETRY,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 10, no. 3, pp. 198–207, 2012, [Online]. Available: https://sid.ir/paper/604379/en

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