OPTIMIZATION OF INCUBATION TEMPERATURE IN EMBRYONATED CHICKEN EGGS INOCULATED WITH H9N2 VACCINAL SUBTYPE OF AVIAN INFLUENZA VIRUS

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KHALILI IRAJ; GHADIMIPOUR RAHIM; AMEGHI ALI; SEDIGH ETEGHAD SAEED

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Journal: VETERINARY RESEARCH FORUM Year:2013 | Volume:4 | Issue:3 Page(s): 145-148

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Title

OPTIMIZATION OF INCUBATION TEMPERATURE IN EMBRYONATED CHICKEN EGGS INOCULATED WITH H9N2 VACCINAL SUBTYPE OF AVIAN INFLUENZA VIRUS

Author(s)

KHALILI IRAJ | GHADIMIPOUR RAHIM | AMEGHI ALI | SEDIGH ETEGHAD SAEED | Issue Writer Certificate

Keywords

INACTIVATED INFLUENZA VACCINE

INCUBATION TEMPERATURE

OPTIMIZATION

Abstract

There are little information about growth properties of low pathogenic (LP) avian influenza virus (AIV) in embryonated chicken eggs (ECEs) at different INCUBATION TEMPERATUREs. Knowledge of this information increases the quantity and quality of antigen in vaccine production process. For this purpose, 10-5 dilution of AIV (A/Chicken/Iran/99/H9N2) was inoculated (Intra-allantoic) into 400, 11-day old specific pathogen free (SPF) ECEs in the 0.1 mL per ECE rate and incubated in 32, 33, 34, 35, 36, 37.5, 38, 39oC for 72 hr in 65% humidity. Early death embryos in first 24 hr were removed. Amnio-allantoic fluid was withdrawn into the measuring cylinder, and tested for hemagglutination (HA) activity and egg infective dose 50 (EID50). The utilizable ECEs and amnio-allantoic fluid volume was significantly increased in 35oC, (p<0.05). Significant difference in HA and EID50 titers, were seen only in 39oC group. Therefore, 35oC is an optimum temperature for incubation of inoculated ECEs.

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APA: Copy

KHALILI, IRAJ, GHADIMIPOUR, RAHIM, AMEGHI, ALI, & SEDIGH ETEGHAD, SAEED. (2013). OPTIMIZATION OF INCUBATION TEMPERATURE IN EMBRYONATED CHICKEN EGGS INOCULATED WITH H9N2 VACCINAL SUBTYPE OF AVIAN INFLUENZA VIRUS. VETERINARY RESEARCH FORUM, 4(3), 145-148. SID. https://sid.ir/paper/616415/en

Vancouver: Copy

KHALILI IRAJ, GHADIMIPOUR RAHIM, AMEGHI ALI, SEDIGH ETEGHAD SAEED. OPTIMIZATION OF INCUBATION TEMPERATURE IN EMBRYONATED CHICKEN EGGS INOCULATED WITH H9N2 VACCINAL SUBTYPE OF AVIAN INFLUENZA VIRUS. VETERINARY RESEARCH FORUM[Internet]. 2013;4(3):145-148. Available from: https://sid.ir/paper/616415/en

IEEE: Copy

IRAJ KHALILI, RAHIM GHADIMIPOUR, ALI AMEGHI, and SAEED SEDIGH ETEGHAD, “OPTIMIZATION OF INCUBATION TEMPERATURE IN EMBRYONATED CHICKEN EGGS INOCULATED WITH H9N2 VACCINAL SUBTYPE OF AVIAN INFLUENZA VIRUS,” VETERINARY RESEARCH FORUM, vol. 4, no. 3, pp. 145–148, 2013, [Online]. Available: https://sid.ir/paper/616415/en

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