Spatial Expression of Genes in Inulin Biosynthesis Pathway in Wild and Root Type Chicory

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SHOORIDEH H.; PEIGHAMBARI S.A.; OMIDI M.; NAGHAVI M.R.; MAROUFI A.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST) Year:2018 | Volume:20 | Issue:5 Page(s): 1049-1058

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

580

Download:

219

Cites:

Information Journal Paper

Title

Spatial Expression of Genes in Inulin Biosynthesis Pathway in Wild and Root Type Chicory

Author(s)

Keywords

Industrial ChicoryQ3

Real Time PCR-FructanQ3

1-SST enzyme activityQ3

1-FEH IQ3

Abstract

Chicory (Cichorium intybus L. ) is a typical Mediterranean plant distributed throughout the world and has different commercial uses such as salad, forage, inulin production, and coffee substitute. Health promoting characteristics of inulin as a prebiotic compound led to its biosynthesis pathway discovery. Two enzymes, namely, 1-SST and 1-FFT, are involved in inulin biosynthesis during normal phase. By cold nights or other factors, 1-FEHs enzymes degrade inulin to fructosyl units. To compare the strength of function of these genes in a wild type genotype with root type cultivar (Orchies) of chicory at three stages, i. e. 60, 90, and 120 days after seed planting, relative expression of those genes along with their corresponding metabolites were assessed using RT-qPCR and HPLC. Expression results showed that, unlike Orchies cultivar, relative expression of 1-SST in wild type genotype was ascending, relative expression of 1-FFT was very low and constant and there were high levels of relative expression of 1-FEH I gene during growing season due to flowering initiation. Also, glucose and fructose concentrations were upward, as result of 1-SST and 1-FEH I enzymes activity in wild type genotype, respectively. Degree of Polymerization (DP) of produced inulin had almost no increase due to low function of 1-FFT enzyme in the wild type genotype (DP< 5), but Orchies cultivar produced inulin with DP> 10 as expression of 1-SST decreased during growing season. So, it is possible to make inulin pathway in root type chicory cultivars more efficient by expanding and overexpressing 1-SST function using such wild resources through backcross breeding or biotechnology methods.

Cites

No record.

References

Cite

APA: Copy

PEIGHAMBARI, S.A., OMIDI, M., NAGHAVI, M.R., & MAROUFI, A.. (2018). Spatial Expression of Genes in Inulin Biosynthesis Pathway in Wild and Root Type Chicory. JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST), 20(5 ), 1049-1058. SID. https://sid.ir/paper/63017/en

Vancouver: Copy

PEIGHAMBARI S.A., OMIDI M., NAGHAVI M.R., MAROUFI A.. Spatial Expression of Genes in Inulin Biosynthesis Pathway in Wild and Root Type Chicory. JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST)[Internet]. 2018;20(5 ):1049-1058. Available from: https://sid.ir/paper/63017/en

IEEE: Copy

S.A. PEIGHAMBARI, M. OMIDI, M.R. NAGHAVI, and A. MAROUFI, “Spatial Expression of Genes in Inulin Biosynthesis Pathway in Wild and Root Type Chicory,” JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST), vol. 20, no. 5 , pp. 1049–1058, 2018, [Online]. Available: https://sid.ir/paper/63017/en

Related Journal Papers