OPTIMIZATION OF THE ANALYSIS OF ALMOND DNA SIMPLE SEQUENCE REPEATS (SSRS) THROUGH SUBMARINE ELECTROPHORESIS USING DIFFERENT AGAROSES AND STAINING PROTOCOLS

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RASOULI M.; MOUSAVI A.; MOHAMMAD PARAST B.; MARTINEZ GOMEZ P.

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Journal: Journal of Nuts Year:2012 | Volume:3 | Issue:2 Page(s): 17-20

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Title

OPTIMIZATION OF THE ANALYSIS OF ALMOND DNA SIMPLE SEQUENCE REPEATS (SSRS) THROUGH SUBMARINE ELECTROPHORESIS USING DIFFERENT AGAROSES AND STAINING PROTOCOLS

Author(s)

RASOULI M. | MOUSAVI A. | MOHAMMAD PARAST B. | MARTINEZ GOMEZ P. | Issue Writer Certificate

Keywords

ALMOND

PRUNUS DULCIS

BREEDING

CULTIVAR

FINGERPRINTING

ROUTINE APPLICATION

MOLECULAR MARKERS

Abstract

Simple sequence repeat (SSR markers or microsatellites), based on the specific PCR amplification of DNA sequences, are becoming the markers of choice for molecular characterization of a wide range of plants because of their high polymorphism, abundance, and codominant inheritance. Different methods have been used for the analysis of the SSR amplified fragments being submarine agarose electrophoresis the more suitable method for the ROUTINE APPLICATION. In this work we have performed a comparative study of the utilization of four different types of low melting (Metaphor®, Sea Kem®, and MS-8®) and regular (LD-2®) agaroses and two different staining protocols using Ethidium Bromide and Gel Red Nucleic Acid Gel Sating®. ALMOND CULTIVARs assayed included the Spanish CULTIVARs ‘Antoneta’, ‘Marta’, ‘Penta’, ‘Tardona’ ‘Desmayo’ and ‘Guara’, the French CULTIVARs ‘Ferragnes’ and ‘R1000’, the USA CULTIVAR ‘Mission’, the Tunisian CULTIVAR ‘Achaak’, the Italian CULTIVAR ‘Tuono’ and the Australian CULTIVAR ‘Chellaston’. SSR detection using Metaphor® agarose gel electrophoresis was the most efficient with higher resolution and would be able to resolve most of allelic variation in comparison with the other three agaroses assayed. In addition, gel staining using Ethidium Bromide showed similar results than the GelRedTM Nucleic Acid Gel Stain® although it is much more toxic. The use of MetaPhor® agarose and GelRedTM Nucleic Acid Gel Stain® appears good indicated for molecular characterization of mapping of population due to its good resolution in comparison with the rest of agaroses, less toxicity in comparison with the use of Ethidium Bromide, and lower cost and easier ROUTINE APPLICATION in comparison with the automatic capillary sequencing.

Cites

IDENTIFICATION OF SELF- INCOMPATIBILITY ALLELES IN SOME ALMOND GENOTYPES BY DEGENERATE S-RNASE PRIMERS

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APA: Copy

RASOULI, M., MOUSAVI, A., MOHAMMAD PARAST, B., & MARTINEZ GOMEZ, P.. (2012). OPTIMIZATION OF THE ANALYSIS OF ALMOND DNA SIMPLE SEQUENCE REPEATS (SSRS) THROUGH SUBMARINE ELECTROPHORESIS USING DIFFERENT AGAROSES AND STAINING PROTOCOLS. JOURNAL OF NUTS (INTERNATIONAL JOURNAL OF NUTS AND RELATED SCIENCES), 3(2), 17-20. SID. https://sid.ir/paper/634500/en

Vancouver: Copy

RASOULI M., MOUSAVI A., MOHAMMAD PARAST B., MARTINEZ GOMEZ P.. OPTIMIZATION OF THE ANALYSIS OF ALMOND DNA SIMPLE SEQUENCE REPEATS (SSRS) THROUGH SUBMARINE ELECTROPHORESIS USING DIFFERENT AGAROSES AND STAINING PROTOCOLS. JOURNAL OF NUTS (INTERNATIONAL JOURNAL OF NUTS AND RELATED SCIENCES)[Internet]. 2012;3(2):17-20. Available from: https://sid.ir/paper/634500/en

IEEE: Copy

M. RASOULI, A. MOUSAVI, B. MOHAMMAD PARAST, and P. MARTINEZ GOMEZ, “OPTIMIZATION OF THE ANALYSIS OF ALMOND DNA SIMPLE SEQUENCE REPEATS (SSRS) THROUGH SUBMARINE ELECTROPHORESIS USING DIFFERENT AGAROSES AND STAINING PROTOCOLS,” JOURNAL OF NUTS (INTERNATIONAL JOURNAL OF NUTS AND RELATED SCIENCES), vol. 3, no. 2, pp. 17–20, 2012, [Online]. Available: https://sid.ir/paper/634500/en

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