EFFECT OF FOLLICULAR SIZE ONIN VITRO MATURATION, FERTILIZATION AND CULTURE OF SHEEP EMBRYOS

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WANI A.R.; KHAN M.Z.; SOFI K.A.; MALIK A.A.; LONE F.A.; BHAT F.A.

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Journal: IRANIAN JOURNAL OF VETERINARY RESEARCH Year:2013 | Volume:14 | Issue:4 (45) Page(s): 299-304

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Title

EFFECT OF FOLLICULAR SIZE ONIN VITRO MATURATION, FERTILIZATION AND CULTURE OF SHEEP EMBRYOS

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Keywords

CLEAVAGE

FOLLICULAR SIZE

IN VITRO MATURATION

IN VITRO FERTILIZATION

SHEEP

Abstract

A study was conducted to investigate the effect of follicular size (small, <2 mm diameter and large, ≥2 mm) onin vitro maturation, fertilization and culture of ovine oocytes. The ovaries (n=80) were collected from abattoirs in Srinagar city, India and transported in normal saline solution containing 50 mg gentamicin sulphate per ml in a thermos flask. The oocytes were collected by puncture technique and good and fair quality oocytes obtained were subjected to maturation in TCM-199 supplemented with epidermal growth factor (EGF) for 24 h under 5% CO2 and humidified atmosphere at 38.5oC. The matured oocytes were then subsequently subjected to fertilization and culturing was done for six days. The results revealed that the maturation rate of large follicle oocytes (85.20%) was higher than small follicle oocytes (52.44%, P=0.002).In vitrofertilization rates for small and large follicle oocytes were not significantly different (79.54 and 76.65%, respectively). However, cleavage and morula rates were higher in large follicle oocytes (58.69 and 34.13%) than small follicle oocytes (38.63 and 19.32%, respectively, P<0.05). In conclusion, there is higher developmental competence of oocytes from the large follicles than small follicles and the pre-maturation of oocyte occurring during follicular growth is essential for subsequent embryonic development in sheep.

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APA: Copy

WANI, A.R., KHAN, M.Z., SOFI, K.A., MALIK, A.A., LONE, F.A., & BHAT, F.A.. (2013). EFFECT OF FOLLICULAR SIZE ONIN VITRO MATURATION, FERTILIZATION AND CULTURE OF SHEEP EMBRYOS. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), 14(4 (45)), 299-304. SID. https://sid.ir/paper/673278/en

Vancouver: Copy

WANI A.R., KHAN M.Z., SOFI K.A., MALIK A.A., LONE F.A., BHAT F.A.. EFFECT OF FOLLICULAR SIZE ONIN VITRO MATURATION, FERTILIZATION AND CULTURE OF SHEEP EMBRYOS. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR)[Internet]. 2013;14(4 (45)):299-304. Available from: https://sid.ir/paper/673278/en

IEEE: Copy

A.R. WANI, M.Z. KHAN, K.A. SOFI, A.A. MALIK, F.A. LONE, and F.A. BHAT, “EFFECT OF FOLLICULAR SIZE ONIN VITRO MATURATION, FERTILIZATION AND CULTURE OF SHEEP EMBRYOS,” IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), vol. 14, no. 4 (45), pp. 299–304, 2013, [Online]. Available: https://sid.ir/paper/673278/en

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