PROKARYOTIC EXPRESSION, PURIFICATION, AND POLYCLONAL ANTIBODY PRODUCTION OF A TRUNCATED RECOMBINANT RABIES VIRUS L PROTEIN

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ZHANG JINYANG; JIN ZIAN; SUN TAO; JIANG YAN; HAN QINQIN; SONG YUZHU; CHEN QIANG; XIA XUESHAN

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Journal Paper

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Journal: Iranian Journal of Biotechnology Year:2015 | Volume:13 | Issue:2 Page(s): 18-24

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Title

PROKARYOTIC EXPRESSION, PURIFICATION, AND POLYCLONAL ANTIBODY PRODUCTION OF A TRUNCATED RECOMBINANT RABIES VIRUS L PROTEIN

Author(s)

Keywords

L PROTEIN

POLYCLONAL ANTIBODY

PROKARYOTIC EXPRESSION

RABIES VIRUS

Abstract

Background: RABIES VIRUS (RABV) is a deadly neurotropic virus that causes the disease of rabies in humans and animals.L PROTEIN is one of the large structuraL PROTEIN of RABIES VIRUS, which displays multiple enzymatic activities, and is required for viral transcription and replication.Objectives: A truncated L PROTEIN of RABIES VIRUS is being cloned, expressed and purified to produce relevant POLYCLONAL ANTIBODY.Materials and Methods: The gene fragment of L PROTEIN of RABV was subcloned into PROKARYOTIC EXPRESSION vector pET-28a and transformed intoE. coli Rosetta DE3 host strain. The recombinant L PROTEIN of RABV was expressed and characterized by SDS-PAGE and western blot analysis using anti-his tag antibody. Mice were immunized with the purified recombinant L PROTEIN, the reaction of the anti-serum was checked by immunofluorescence and dot-blot, respectively.Results: The results of PCR and sequencing confirmed that the fragment of L gene of RABV was successfully cloned into the expression vector. The expression of recombinant L PROTEIN fragment induced by IPTG was confirmed by the band of 43 kDa in SDS-PAGE and western blot. The antiserum of purified L PROTEIN immunized mice was reacted with RABV infected N2a cells and suckling mouse brain tissue lysates.Conclusions: Our data showed that the recombinant L PROTEIN produced by pET-28a vector was very successful, and the purified L PROTEIN could efficiently induce the antibody response in mice. The antiserum could recognize the virus in RABV infected cells and tissue very well.

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APA: Copy

ZHANG, JINYANG, JIN, ZIAN, SUN, TAO, JIANG, YAN, HAN, QINQIN, SONG, YUZHU, CHEN, QIANG, & XIA, XUESHAN. (2015). PROKARYOTIC EXPRESSION, PURIFICATION, AND POLYCLONAL ANTIBODY PRODUCTION OF A TRUNCATED RECOMBINANT RABIES VIRUS L PROTEIN. IRANIAN JOURNAL OF BIOTECHNOLOGY, 13(2), 18-24. SID. https://sid.ir/paper/688664/en

Vancouver: Copy

ZHANG JINYANG, JIN ZIAN, SUN TAO, JIANG YAN, HAN QINQIN, SONG YUZHU, CHEN QIANG, XIA XUESHAN. PROKARYOTIC EXPRESSION, PURIFICATION, AND POLYCLONAL ANTIBODY PRODUCTION OF A TRUNCATED RECOMBINANT RABIES VIRUS L PROTEIN. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2015;13(2):18-24. Available from: https://sid.ir/paper/688664/en

IEEE: Copy

JINYANG ZHANG, ZIAN JIN, TAO SUN, YAN JIANG, QINQIN HAN, YUZHU SONG, QIANG CHEN, and XUESHAN XIA, “PROKARYOTIC EXPRESSION, PURIFICATION, AND POLYCLONAL ANTIBODY PRODUCTION OF A TRUNCATED RECOMBINANT RABIES VIRUS L PROTEIN,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 13, no. 2, pp. 18–24, 2015, [Online]. Available: https://sid.ir/paper/688664/en

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