ENHANCEMENT OF SOLUBILITY AND SPECIFIC ACTIVITY OF A CU/ZN SUPEROXIDE DISMUTASE BY CO-EXPRESSION WITH A COPPER CHAPERONE IN ESCHERICHIA COLI

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EIAMPHUNGPORN WARAWAN; YAINOY SAKDA; PRACHAYASITTIKUL VIRAPONG

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Journal Paper

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Journal: Iranian Journal of Biotechnology Year:2016 | Volume:14 | Issue:4 Page(s): 243-249

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Title

ENHANCEMENT OF SOLUBILITY AND SPECIFIC ACTIVITY OF A CU/ZN SUPEROXIDE DISMUTASE BY CO-EXPRESSION WITH A COPPER CHAPERONE IN ESCHERICHIA COLI

Author(s)

EIAMPHUNGPORN WARAWAN | YAINOY SAKDA | PRACHAYASITTIKUL VIRAPONG | Issue Writer Certificate

Keywords

CU/ZN SUPEROXIDE DISMUTASE

CO-EXPRESSION

HUMAN COPPER CHAPERONE

Abstract

Background: Human CU/ZN SUPEROXIDE DISMUTASE (hSOD1) is an antioxidant enzyme with potential as a therapeutic agent. However, heterologous expression of hSOD1 has remained an issue due to Cu2+insufficiency at protein active site, leading to low solubility and enzymatic activity.Objectives: The effect of co-expressed HUMAN COPPER CHAPERONE (hCCS) to enhance the solubility and enzymatic activity of hSOD1 inE. coli was investigated in the presence and absence of Cu2+.Materials and Methods: pETDuet-1-hSOD1 and pETDuet-1-hCCS-hSOD1 were constructed and individually transformed intoE. coli strain BL21 (DE3). The recombinant hSOD1 was expressed and purified using immobilized metal affinity chromatography. The yield and specific activity of hSOD1 in all conditions were studied.Results: CO-EXPRESSION with hCCS increased hSOD1 solubility at 37 o C, but this effect was not observed at 25 o C. Notably, the specific activity of hSOD1 was enhanced by 1.5 fold and greater than 3 fold when co-expressed with hCCS at 25o C with and without Cu2+supplement, respectively. However, the chaperone CO-EXPRESSION did not significantly increase the yield of hSOD1 comparable to the expression of hSOD1 alone.Conclusions: This study is the first report demonstrating a potential use of hCCS for heterologous production of hSOD1 with high enzymatic activity.

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APA: Copy

EIAMPHUNGPORN, WARAWAN, YAINOY, SAKDA, & PRACHAYASITTIKUL, VIRAPONG. (2016). ENHANCEMENT OF SOLUBILITY AND SPECIFIC ACTIVITY OF A CU/ZN SUPEROXIDE DISMUTASE BY CO-EXPRESSION WITH A COPPER CHAPERONE IN ESCHERICHIA COLI. IRANIAN JOURNAL OF BIOTECHNOLOGY, 14(4), 243-249. SID. https://sid.ir/paper/692221/en

Vancouver: Copy

EIAMPHUNGPORN WARAWAN, YAINOY SAKDA, PRACHAYASITTIKUL VIRAPONG. ENHANCEMENT OF SOLUBILITY AND SPECIFIC ACTIVITY OF A CU/ZN SUPEROXIDE DISMUTASE BY CO-EXPRESSION WITH A COPPER CHAPERONE IN ESCHERICHIA COLI. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2016;14(4):243-249. Available from: https://sid.ir/paper/692221/en

IEEE: Copy

WARAWAN EIAMPHUNGPORN, SAKDA YAINOY, and VIRAPONG PRACHAYASITTIKUL, “ENHANCEMENT OF SOLUBILITY AND SPECIFIC ACTIVITY OF A CU/ZN SUPEROXIDE DISMUTASE BY CO-EXPRESSION WITH A COPPER CHAPERONE IN ESCHERICHIA COLI,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 14, no. 4, pp. 243–249, 2016, [Online]. Available: https://sid.ir/paper/692221/en

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