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Information Journal Paper

Title

STIMULATION OF CAMEL POLYCLONAL ANTIBODY AGAINST HUMAN T CELL IMMUNOGLOBULIN AND MUCIN 3

Pages

  166-171

Abstract

 Background: T cell Immunoglobulin, Mucin (TIM) -3, is a type I trans membrane glycoprotein belonging to TIM family. This receptor expresses on T helper type 1 (Th1) cells that binds to galectin-9 (Gal9); inducing an inhibitory signal. As a result, apoptosis of Th1 cells occurs and cytotoxicity of CD8 T cells becomes evident in vitro. Therefore, this immunomodulatory molecule may be used as a novel target for clinical purposes. The production of camel polyclonal antibodies against TIM-3-expressing cell line was the purpose of this study.Objectives: In this study, we aimed to use HEK 293 cells expressing human TIM-3 to obtain camel polyclonal antibody against TIM-3 by immunization.Materials and Methods: A pre-synthesized human TIM-3cDNA was inserted into pcDNA3.1 plasmid and the new construct was transfected in HEK cell. TIM-3 expression was confi rmed by qRT-PCR and fl ow cytometry. A camel (6 months old) was immunized with the lysate prepared from rTIM-3 expressing HEK cells 4 times. The anti-TIM-3 antibody level was evaluated using ELISA method.Results: TIM-3 was successfully cloned in HEK cells with 88% success rate. High level of anti-TIM-3 antibody was detected in the serum of the camel immunized with the recombinant cell lysate, after fi nal injection.Conclusions: Our rhTIM-3 cell display system can be useful for future diagnostic or therapeutic approaches.

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    APA: Copy

    HOMAYOUNI, VIDA, KHANAHMAD, HOSSEIN, GANJALIKHANI HAKEMI, MAZDAK, BEHDANI, MAHDI, GHASEMI, POURIA, & REZAEI, ABBAS. (2017). STIMULATION OF CAMEL POLYCLONAL ANTIBODY AGAINST HUMAN T CELL IMMUNOGLOBULIN AND MUCIN 3. IRANIAN JOURNAL OF BIOTECHNOLOGY, 15(3), 166-171. SID. https://sid.ir/paper/693029/en

    Vancouver: Copy

    HOMAYOUNI VIDA, KHANAHMAD HOSSEIN, GANJALIKHANI HAKEMI MAZDAK, BEHDANI MAHDI, GHASEMI POURIA, REZAEI ABBAS. STIMULATION OF CAMEL POLYCLONAL ANTIBODY AGAINST HUMAN T CELL IMMUNOGLOBULIN AND MUCIN 3. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2017;15(3):166-171. Available from: https://sid.ir/paper/693029/en

    IEEE: Copy

    VIDA HOMAYOUNI, HOSSEIN KHANAHMAD, MAZDAK GANJALIKHANI HAKEMI, MAHDI BEHDANI, POURIA GHASEMI, and ABBAS REZAEI, “STIMULATION OF CAMEL POLYCLONAL ANTIBODY AGAINST HUMAN T CELL IMMUNOGLOBULIN AND MUCIN 3,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 15, no. 3, pp. 166–171, 2017, [Online]. Available: https://sid.ir/paper/693029/en

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