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Information Journal Paper

Title

DESIGNING AND VALIDATION OF ONE-STEP T-ARMS-PCR FOR GENOTYPING THE ENOS RS1799983 SNP

Pages

  208-212

Keywords

ENDOTHELIAL NITRIC OXIDE SYNTHASE (ENOS) 

Abstract

 Background: The trans version of G to T (G894T) in human endothelial nitric oxide synthase (eNOS) gene has profound eff ects such as male infertility, recurrent miscarriage, multiple sclerosis and cardiovascular diseases.Objectives: Development of a new Multiplex Tetra-Primer Amplifi cation Refractory Mutation System - Polymerase Chain Reaction (T-ARMS-PCR) for detection of RS1799983 (G894T) in the humaneNOS was sought.Materials and Methods: A T-ARMS-PCR for RS1799983 polymorphism in a single-step PCR was carried out, and the results were confi rmed by PCR-RFLP technique in 82 infertile men with VARICOCELE.Results: The results showed that GG (varicocele infertile men), GT and TT genotypes appear to be 53.65%, 34.14%, and 12.19%, respectively. Full accordance between PCR-RFLP and T-ARMS-PCR methods for genotyping of RS1799983 polymorphism was found.Conclusions: This is the fi rst work that describes a rapid, relatively cheap, high throughput detection of G894T polymorphism ineNOS that can be used in large scale clinical studies.

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    APA: Copy

    HEIDARI, MOHAMMAD MEHDI, & KHATAMI, MEHRI. (2017). DESIGNING AND VALIDATION OF ONE-STEP T-ARMS-PCR FOR GENOTYPING THE ENOS RS1799983 SNP. IRANIAN JOURNAL OF BIOTECHNOLOGY, 15(3), 208-212. SID. https://sid.ir/paper/693959/en

    Vancouver: Copy

    HEIDARI MOHAMMAD MEHDI, KHATAMI MEHRI. DESIGNING AND VALIDATION OF ONE-STEP T-ARMS-PCR FOR GENOTYPING THE ENOS RS1799983 SNP. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2017;15(3):208-212. Available from: https://sid.ir/paper/693959/en

    IEEE: Copy

    MOHAMMAD MEHDI HEIDARI, and MEHRI KHATAMI, “DESIGNING AND VALIDATION OF ONE-STEP T-ARMS-PCR FOR GENOTYPING THE ENOS RS1799983 SNP,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 15, no. 3, pp. 208–212, 2017, [Online]. Available: https://sid.ir/paper/693959/en

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