CLONING AND EXPRESSION OF IRANIAN TURKMEN-THOROUGHBRED HORSE FOLLICLE STIMULATING HORMONE IN PICHIA PASTORIS

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ELYASI GORJI ZAHRA; AMIRI YEKTA AMIR; GOURABI HAMID; HASSANI SAEED; FATEMI NAYERALSADAT; ZEREHDARAN SAEED; VAKHSHITEH FAEZEH; SANATI MOHAMMAD HOSSEIN

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Journal: Iranian Journal of Biotechnology Year:2015 | Volume:13 | Issue:2 Page(s): 10-17

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Title

CLONING AND EXPRESSION OF IRANIAN TURKMEN-THOROUGHBRED HORSE FOLLICLE STIMULATING HORMONE IN PICHIA PASTORIS

Author(s)

Keywords

FOLLICLE STIMULATING HORMONE

HORSE

MOLECULAR CLONING

P. PASTORIS

Abstract

Background: FOLLICLE STIMULATING HORMONE (FSH) plays an essential role in reproductive physiology and follicular development.Objective: A new variant of the equine fsh (efsh) gene was cloned, sequenced, and expressed in Pichia pastoris (P. pastoris) GS115 yeast expression system.Materials and Methods: The full-length cDNAs of the efshα and efsh β chains were amplified by reverse transcription polymerase chain reaction (RT-PCR) using the total RNA isolated from an Iranian Turkmen-thoroughbred HORSE’s anterior pituitary gland. The amplifiedefsh chains were cloned into the pPIC9 vector and transferred into P. PASTORIS. The secretion of recombined eFSH using P. PASTORIS expression system was confirmed by Western blotting and immunoprecipitation (IP) methods.Results: The DNA sequence of the efsh b chain accession number JX861871, predicted two putative differential nucleotide arrays, both of which are located in the 3′UTR. Western blotting showed a molecular mass of 13 and 18 kDa for eFSHαand eFSH b subunits, respectively. The expression of desired protein was confirmed by protein G immunoprecipitation kit.Conclusions: eFSH successfully expressed in P. PASTORIS. These findings lay a foundation to improve ovulation and embryo recovery rates as well as the efficiency of total embryo-transfer process in mares.

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APA: Copy

ELYASI GORJI, ZAHRA, AMIRI YEKTA, AMIR, GOURABI, HAMID, HASSANI, SAEED, FATEMI, NAYERALSADAT, ZEREHDARAN, SAEED, VAKHSHITEH, FAEZEH, & SANATI, MOHAMMAD HOSSEIN. (2015). CLONING AND EXPRESSION OF IRANIAN TURKMEN-THOROUGHBRED HORSE FOLLICLE STIMULATING HORMONE IN PICHIA PASTORIS. IRANIAN JOURNAL OF BIOTECHNOLOGY, 13(2), 10-17. SID. https://sid.ir/paper/695855/en

Vancouver: Copy

ELYASI GORJI ZAHRA, AMIRI YEKTA AMIR, GOURABI HAMID, HASSANI SAEED, FATEMI NAYERALSADAT, ZEREHDARAN SAEED, VAKHSHITEH FAEZEH, SANATI MOHAMMAD HOSSEIN. CLONING AND EXPRESSION OF IRANIAN TURKMEN-THOROUGHBRED HORSE FOLLICLE STIMULATING HORMONE IN PICHIA PASTORIS. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2015;13(2):10-17. Available from: https://sid.ir/paper/695855/en

IEEE: Copy

ZAHRA ELYASI GORJI, AMIR AMIRI YEKTA, HAMID GOURABI, SAEED HASSANI, NAYERALSADAT FATEMI, SAEED ZEREHDARAN, FAEZEH VAKHSHITEH, and MOHAMMAD HOSSEIN SANATI, “CLONING AND EXPRESSION OF IRANIAN TURKMEN-THOROUGHBRED HORSE FOLLICLE STIMULATING HORMONE IN PICHIA PASTORIS,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 13, no. 2, pp. 10–17, 2015, [Online]. Available: https://sid.ir/paper/695855/en

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