ANTI-VASCULOGENIC ACTIVITY OF A POLYSACCHARIDE DERIVED FROM BRITTLE STAR VIA INHIBITION OF VEGF, PAXILLIN AND MMP-9

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BAHARARA JAVAD; AMINI ELAHEH; MUSAVI MARZIYEH

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Journal: Iranian Journal of Biotechnology Year:2017 | Volume:15 | Issue:3 Page(s): 179-185

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Title

ANTI-VASCULOGENIC ACTIVITY OF A POLYSACCHARIDE DERIVED FROM BRITTLE STAR VIA INHIBITION OF VEGF, PAXILLIN AND MMP-9

Author(s)

BAHARARA JAVAD | AMINI ELAHEH | MUSAVI MARZIYEH | Issue Writer Certificate

Keywords

ANGIOGENESIS

CHICK CHORIOALLANTOIC MEMBRANE

ENDOTHELIAL CELLS

MMP-9

VEGF

Abstract

Background: Bioactive compounds such as terpenoids, chondroitin sulfate, and polysaccharides with added value can be found in prestine marine creatures. These compounds often do have highly valuable therapeutic applications such as being antioxidant, antitumoro genic, anti-infl ammatory and anti-angiogenic. For the latter, varieties of ANGIOGENESIS factors can suppress this issue within the bodily tissues.Objectives: The anti-angiogenic and anti-metastatic capacity of a polysaccharide derived from brittle star was investigated.Material and Methods: The anti-proliferative eff ect of derived polysaccharide on umbilical vein ENDOTHELIAL CELLS (HUVEC) was measured using MTT (dimethyl thiazol-2-yl] -2, 5-diphenyl tetrazolium bromide) assay. The anti-angiogenic eff ect of the isolated polysaccharide was examined by Chorioallantoic membrane (CAM) assay. The transcriptional expression of VEGF (Vascular Endothelial Growth Factor) was evaluated by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). The anti-metastatic activity was investigated via scratch-wound healing assay. The levels of Paxillin and Matrix Metalloproteinase-9 (MMP-9) expression were analyzed by RT-PCR. Statistical analysis and mean comparisons (p<0.05) were carried out by SPSS 16.Results: Our results elucidated that the brittle star isolated polysaccharide exerted a dose dependent cytotoxic eff ect on the HUVEC ENDOTHELIAL CELLS. The CAM assay exhibited potent anti-angiogenic activityin vivo. The RT-PCR analysis showed that the extracted polysaccharide (40, 60 mg.mL-1) down-regulated the VEGF expression. Further, the diminished attachment of ENDOTHELIAL CELLS demonstrated that the anti-invasiveness of the derived polysaccharide (25, 50 mg.mL-1) was administrated via down-regulation of paxillin and MMP-9 mRNA expression.Conclusions: Taken together, these results indicated that the polysaccharide extracted from brittle star was able to decrease the viability of the HUVEC cells, to suppress ANGIOGENESIS, and possibly act as a natural anti-angiogenic and anti-metastatic marine organic compound against ANGIOGENESIS related pathologies.

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APA: Copy

BAHARARA, JAVAD, AMINI, ELAHEH, & MUSAVI, MARZIYEH. (2017). ANTI-VASCULOGENIC ACTIVITY OF A POLYSACCHARIDE DERIVED FROM BRITTLE STAR VIA INHIBITION OF VEGF, PAXILLIN AND MMP-9. IRANIAN JOURNAL OF BIOTECHNOLOGY, 15(3), 179-185. SID. https://sid.ir/paper/695908/en

Vancouver: Copy

BAHARARA JAVAD, AMINI ELAHEH, MUSAVI MARZIYEH. ANTI-VASCULOGENIC ACTIVITY OF A POLYSACCHARIDE DERIVED FROM BRITTLE STAR VIA INHIBITION OF VEGF, PAXILLIN AND MMP-9. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2017;15(3):179-185. Available from: https://sid.ir/paper/695908/en

IEEE: Copy

JAVAD BAHARARA, ELAHEH AMINI, and MARZIYEH MUSAVI, “ANTI-VASCULOGENIC ACTIVITY OF A POLYSACCHARIDE DERIVED FROM BRITTLE STAR VIA INHIBITION OF VEGF, PAXILLIN AND MMP-9,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 15, no. 3, pp. 179–185, 2017, [Online]. Available: https://sid.ir/paper/695908/en

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