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Information Journal Paper

Title

EXPRESSION PROFI LING OF HSPB1 AND TP53 GENES THROUGH RT-QPCR IN DIFF ERENT CANCER TYPES OF CANIS FAMILIARIS

Pages

  186-193

Abstract

 Background: Diagnostic molecular marker studies are in vogue to have insight of most prevalent animal diseases including cancer.Objectives: Gene expression profi ling of pro and anti-apoptotic genes was conducted in dog Lymphoma, CTVT, SCC, granuloma, perianal adenocarcinoma and mammary tumors.Materials and Methods: Cancerous tissues of 21 aff ected animals were obtained. Total RNA was extracted followed by cDNA synthesis. Comparative Ct method viaTaqman assay (RT-qPCR) was used to quantify corresponding mRNA molecules, TP53 and HSPB1, as normalized by GAPDH as the reference gene.Results: HSPB1 showed ectopic expression in lymphoma, CTVT and mammary tumors; its down-regulation was observed in granuloma and oral SCC with fold diff erence (FD) of ±35. Similarly, TP53 as the tumor suppressor gene with proapoptotic properties, showed up-regulation in all tumor types, notably 80% of mammary tumors and 60% of CTVT. The FD values were 33.31 and 2.27, respectively.Conclusion: Altered transcriptomic response of HSPB1 and TP53 was observed in all cancer types of Canis familiaris. The resulting profi le depicts the involvement of the genes in cancer pathways. Thus, the data might be helpful for diagnosis, prognosis, identifi cation and classifi cation of these widespread neoplasms in this species.

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    APA: Copy

    SAIF, RASHID, AWAN, ALI RAZA, TAYYAB, MUHAMMAD, ELLAHI BABAR, MASROOR, MAHMOOD, ASIM KHALID, ULLAH, ZIA, ZIA, SAEEDA, & WASIM, MUHAMMAD. (2017). EXPRESSION PROFI LING OF HSPB1 AND TP53 GENES THROUGH RT-QPCR IN DIFF ERENT CANCER TYPES OF CANIS FAMILIARIS. IRANIAN JOURNAL OF BIOTECHNOLOGY, 15(3), 186-193. SID. https://sid.ir/paper/699006/en

    Vancouver: Copy

    SAIF RASHID, AWAN ALI RAZA, TAYYAB MUHAMMAD, ELLAHI BABAR MASROOR, MAHMOOD ASIM KHALID, ULLAH ZIA, ZIA SAEEDA, WASIM MUHAMMAD. EXPRESSION PROFI LING OF HSPB1 AND TP53 GENES THROUGH RT-QPCR IN DIFF ERENT CANCER TYPES OF CANIS FAMILIARIS. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2017;15(3):186-193. Available from: https://sid.ir/paper/699006/en

    IEEE: Copy

    RASHID SAIF, ALI RAZA AWAN, MUHAMMAD TAYYAB, MASROOR ELLAHI BABAR, ASIM KHALID MAHMOOD, ZIA ULLAH, SAEEDA ZIA, and MUHAMMAD WASIM, “EXPRESSION PROFI LING OF HSPB1 AND TP53 GENES THROUGH RT-QPCR IN DIFF ERENT CANCER TYPES OF CANIS FAMILIARIS,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 15, no. 3, pp. 186–193, 2017, [Online]. Available: https://sid.ir/paper/699006/en

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