EFFECT OF RESISTANCE FACTOR TRANSMISSION IN E.COLI STRAINS ISOLATED OF URINARY TRACT INFECTIONS

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PEIRAVY F.; RAFIEI TABATABAEI R.

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Journal: JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY) Year:2006 | Volume:15 | Issue:58/1 (BIOLOGY) Page(s): 505-515

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Title

EFFECT OF RESISTANCE FACTOR TRANSMISSION IN E.COLI STRAINS ISOLATED OF URINARY TRACT INFECTIONS

Author(s)

PEIRAVY F. | RAFIEI TABATABAEI R. | Issue Writer Certificate

Keywords

RESISTANCE FACTOR TRANSMISSIONQ4

E. COLIQ3

URINARY TRACT INFECTIONQ4

Abstract

65 E.coli strains isolated from cases of URINARY TRACT INFECTIONs from Qaemshahr and Sari hospitals in 1383 were analyzed to determine their drug resistance transfer factor. Isolation and identification of strains were carried out using standard procedures- Antimicrobial sensivity tests was carried out using single disk-diffusion method. The highest rate of resistance was against Ampicillin (87.7%) followed by Tetracycline (73.8%) and Cephalotin (61.5%). 75% of strains showed resistance to three or more antibiotics (MDR=Multiple Drug Resistance). MIC was carried out using Macrodilution method MIC of Nalidixic acid for all strains susceptible against this antibiotic and MIC of alt drugs used in this study for recipient strain was determined. Conjugation was performed using mixed culture method to determine Resistance transfer factor. E.coii DH5aF-lac+Na+r used as recipient strain. 60% of E.coli strains susceptible to Nalidixic acid had transferable resistance factor. Resistance to tetracycline had The highest rate of transfer (45%) followed by ampicillin (37.1%) chloramphenicol (33.3%), Trimethoprim - Sulfamethoxazole (23.1 %).The lowest rate of transfer was for cephalotin (7.1 %). In our study E.coli Isolates showed high percentage of resistance to drug used. These data confirms that significant increase in appearance of drug resistant strains is due to uncontrolled use of antibiotics in human and animals, and resistance transfer among different bacteria. To limit using antibiotics in human and animals, performing antimicrobial susceptibility tests to select a suitable antimicrobial agent, application of recommended dosage regimens and duration of therapy may decline the extent of resistant bacteria.

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APA: Copy

PEIRAVY, F., & RAFIEI TABATABAEI, R.. (2006). EFFECT OF RESISTANCE FACTOR TRANSMISSION IN E.COLI STRAINS ISOLATED OF URINARY TRACT INFECTIONS. JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY), 15(58/1 (BIOLOGY)), 505-515. SID. https://sid.ir/paper/70217/en

Vancouver: Copy

PEIRAVY F., RAFIEI TABATABAEI R.. EFFECT OF RESISTANCE FACTOR TRANSMISSION IN E.COLI STRAINS ISOLATED OF URINARY TRACT INFECTIONS. JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY)[Internet]. 2006;15(58/1 (BIOLOGY)):505-515. Available from: https://sid.ir/paper/70217/en

IEEE: Copy

F. PEIRAVY, and R. RAFIEI TABATABAEI, “EFFECT OF RESISTANCE FACTOR TRANSMISSION IN E.COLI STRAINS ISOLATED OF URINARY TRACT INFECTIONS,” JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY), vol. 15, no. 58/1 (BIOLOGY), pp. 505–515, 2006, [Online]. Available: https://sid.ir/paper/70217/en

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