PRODUCTION OF MARKER-FREE TRANSGENIC RICE (ORYZA SATIVA L.) WITH IMPROVED NUTRITIVE QUALITY EXPRESSING AMA1

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

XU MING; ZHAO SHUAI; YUWEN ZHANG; YIN HENGJIE; PENG XUEJUAN; CHENG ZUXIN; YANG ZHIJIAN; ZHENG JINGUI

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Biotechnology Year:2017 | Volume:15 | Issue:2 Page(s): 0-0

Download Full-Text

View:

289

Download:

Cites:

Information Journal Paper

Title

PRODUCTION OF MARKER-FREE TRANSGENIC RICE (ORYZA SATIVA L.) WITH IMPROVED NUTRITIVE QUALITY EXPRESSING AMA1

Author(s)

Keywords

AMA1 GENE

CO-TRANSFORMATION

ESSENTIAL AMINO ACID

SELECTABLE MARKER-FREE

RICE

Abstract

Background: RICE seed proteins are lacking ESSENTIAL AMINO ACIDs (EAAs). Genetic engineering offers a fast and sustainable method to solve this problem as it allows the specific expression of heterologous EAA-rich proteins. The use of selectable marker gene is essential for generation of transgenic crops, but might also lead to potential environmental and food safety problems. Therefore, the production of marker-free transgenic crops is becoming an extremely attractive alternative and could contribute to the public acceptance of transgenic crops.Objectives: The present study was conducted to examine whether AmA1 can be expressed specifically in RICE seeds, and generate marker-free transgenic RICE with improved nutritive value.Materials and Methods: AmA1 was transferred into RICE using Agrobacterium -mediated CO-TRANSFORMATION system with a twin T-DNA binary vector and its integration in RICE genome was confirmed by southern blot. Transcription ofAmA1 was analyzed by Real-Time PCR and its expression was verified by western analysis. Protein and amino acid content were measured by the Kjeldahl method and the high-speed amino acid analyzer, respectively.Results: Five SELECTABLE MARKER-FREE homozygous transgenic lines were obtained from the progeny. The expression of recombinant AmA1 was confirmed by the observation of a 35 kDa band in SDS-PAGE and western blot. Compared to the wild-type control, the total protein contents in the seeds of fi vehomozygous lines were increased by 1.06~12.87%.In addition, the content of several EAAs, including lysine, threonine, and valine was increased significantly in the best expressing line.Conclusions: The results indicated that the amino acid composition of RICE grain could be improved by seed-specific expression ofAmA1.

Cites

No record.

References

No record.

Cite

APA: Copy

XU, MING, ZHAO, SHUAI, YUWEN, ZHANG, YIN, HENGJIE, PENG, XUEJUAN, CHENG, ZUXIN, YANG, ZHIJIAN, & ZHENG, JINGUI. (2017). PRODUCTION OF MARKER-FREE TRANSGENIC RICE (ORYZA SATIVA L.) WITH IMPROVED NUTRITIVE QUALITY EXPRESSING AMA1. IRANIAN JOURNAL OF BIOTECHNOLOGY, 15(2), 0-0. SID. https://sid.ir/paper/704012/en

Vancouver: Copy

XU MING, ZHAO SHUAI, YUWEN ZHANG, YIN HENGJIE, PENG XUEJUAN, CHENG ZUXIN, YANG ZHIJIAN, ZHENG JINGUI. PRODUCTION OF MARKER-FREE TRANSGENIC RICE (ORYZA SATIVA L.) WITH IMPROVED NUTRITIVE QUALITY EXPRESSING AMA1. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2017;15(2):0-0. Available from: https://sid.ir/paper/704012/en

IEEE: Copy

MING XU, SHUAI ZHAO, ZHANG YUWEN, HENGJIE YIN, XUEJUAN PENG, ZUXIN CHENG, ZHIJIAN YANG, and JINGUI ZHENG, “PRODUCTION OF MARKER-FREE TRANSGENIC RICE (ORYZA SATIVA L.) WITH IMPROVED NUTRITIVE QUALITY EXPRESSING AMA1,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 15, no. 2, pp. 0–0, 2017, [Online]. Available: https://sid.ir/paper/704012/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops