DIFFERENTIAL EXPRESSION OF THE ALPHA S1 CASEIN AND BETA-LACTOGLOBULIN GENES IN DIFFERENT PHYSIOLOGICAL STAGES OF THE ADANI GOATS MAMMARY GLANDS

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MORAMMAZI SALIM; MASOUDI ALI AKBAR; VAEZ TORSHIZI RASOUL; PAKDEL ABBAS

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Journal: Iranian Journal of Biotechnology Year:2016 | Volume:14 | Issue:4 Page(s): 278-285

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Title

DIFFERENTIAL EXPRESSION OF THE ALPHA S1 CASEIN AND BETA-LACTOGLOBULIN GENES IN DIFFERENT PHYSIOLOGICAL STAGES OF THE ADANI GOATS MAMMARY GLANDS

Author(s)

MORAMMAZI SALIM | MASOUDI ALI AKBAR | VAEZ TORSHIZI RASOUL | PAKDEL ABBAS | Issue Writer Certificate

Keywords

ADANI GOATS; ALPHA S1 CASEIN; BETA-LACTOGLOBULIN; GENE EXPRESSION

MAMMARY GLAND

MILK YIELD BREEDING VALUE

Abstract

Background: Milk proteins genes have been the focus of the researches as the candidate target genes that play a decisive role when animal breeding is desired.Objectives: In the present study, the transcriptional levels of Beta-lactoglobulin (BLG) and Alpha S1 casein (CSN1S1) genes were investigated during prenatal, milking and drying times in MAMMARY GLANDs of the Adani goats which showed high and low breeding values.Materials and Methods: The breeding values of the animals were estimated first by applying multi-trait random regression model. Using the biopsy gun, the MAMMARY GLAND samples were taken and real-time PCR was applied to search the expression of the genes. Fixed factors of the model were the breeding value groups, sampling times and their interactions.Results: The interactions were significant for both genes. At milking time, the high breeding value group exhibited more transcriptional levels forBLG and less transcriptional levels for CSN1S1 gene compared with the low breeding value group. The expression patterns of these genes were also different between the two breeding value groups. The maximum level ofBLG and CSN1S1 transcriptions were found to occur at drying time.Conclusions: A difference in the gene expression was observed between the two groups which indicate the change in the nucleotide sequence for transcription factor binding sites, or miRNA binding sites, otherwise in the coding regions.Therefore, the variations in the coding and promoter regions of this gene should be investigated in the further studies.

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APA: Copy

MORAMMAZI, SALIM, MASOUDI, ALI AKBAR, VAEZ TORSHIZI, RASOUL, & PAKDEL, ABBAS. (2016). DIFFERENTIAL EXPRESSION OF THE ALPHA S1 CASEIN AND BETA-LACTOGLOBULIN GENES IN DIFFERENT PHYSIOLOGICAL STAGES OF THE ADANI GOATS MAMMARY GLANDS. IRANIAN JOURNAL OF BIOTECHNOLOGY, 14(4), 278-285. SID. https://sid.ir/paper/704050/en

Vancouver: Copy

MORAMMAZI SALIM, MASOUDI ALI AKBAR, VAEZ TORSHIZI RASOUL, PAKDEL ABBAS. DIFFERENTIAL EXPRESSION OF THE ALPHA S1 CASEIN AND BETA-LACTOGLOBULIN GENES IN DIFFERENT PHYSIOLOGICAL STAGES OF THE ADANI GOATS MAMMARY GLANDS. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2016;14(4):278-285. Available from: https://sid.ir/paper/704050/en

IEEE: Copy

SALIM MORAMMAZI, ALI AKBAR MASOUDI, RASOUL VAEZ TORSHIZI, and ABBAS PAKDEL, “DIFFERENTIAL EXPRESSION OF THE ALPHA S1 CASEIN AND BETA-LACTOGLOBULIN GENES IN DIFFERENT PHYSIOLOGICAL STAGES OF THE ADANI GOATS MAMMARY GLANDS,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 14, no. 4, pp. 278–285, 2016, [Online]. Available: https://sid.ir/paper/704050/en

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