FABRICATION OF A NOVEL HYDROGEN PEROXIDE BIOSENSOR BY IMMOBILIZING MICROPEROXIDASE ON ANHDCT/GC ELECTRODE

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MADHI TAZEHKAND A.; MOHAMMADI B.; ZARGAZI M.H.

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Journal: JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY) Year:2011 | Volume:21 | Issue:80 (CHEMISTRY ISSUE) Page(s): 117-127

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Title

FABRICATION OF A NOVEL HYDROGEN PEROXIDE BIOSENSOR BY IMMOBILIZING MICROPEROXIDASE ON ANHDCT/GC ELECTRODE

Author(s)

MADHI TAZEHKAND A. | MOHAMMADI B. | ZARGAZI M.H. | Issue Writer Certificate

Keywords

MICROPEROXIDASE (MP)Q2

ANHDCT (6-AMINO-5-NITROSO-4-HYDROXY-1Q2

3-DIAZA-5-CYCLOHEXENE-2-THIOONE)Q2

CYCLIC VOLTAMMETRY (CV)Q2

HYDROGEN PEROXIDE (H2O2)Q2

Abstract

Introduction: Enzymes play an important role in the metabolic processes of organisms as akind of typical biological macromolecule and special catalyst. Reversible redox transformation of enzymes at an electrode can be used to drive enzyme-catalyzed reactions.Aim: Here, we study the dirct electrochemical behaviour of MP at ANHDCT / glassy carbon Electrode. MP can display unique bioelectrocatalytical properties in the reduction of H2O2 by direct electron transfer from electron-donating electrod through the active site of the enzyme and to peroxide in solution.Material and Method: MP/ ANHDCT electrode was fabricated by casting the mixture of MP and ANHDCT solutions onto glassy Carbon electrode (GCE). Direct electron transfer process of immobilized MP and its application as a biosensor for H2O2, were investigated by using Cyclic Voltammetry.Results: n This paper is considered to indicat that the MP |ANHDCT| GC electrode undertakes a direct electron transfer reaction and exhibits an excellent electrocatalytic response to the reduction of H2O2 in a Phosphate buffer solution (pH=7). The MP |ANHDCT| GC electrode can be used for determination of H2O2 concentration.Conclusion: The MP / ANHDCT films may have a potential application in constructing the third-generation electrochemical biosensors based on mediator-free electrochemistry of the enzymes. Further work is in progress to extend to other redox enzymebased biosensors.

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APA: Copy

MADHI TAZEHKAND, A., MOHAMMADI, B., & ZARGAZI, M.H.. (2011). FABRICATION OF A NOVEL HYDROGEN PEROXIDE BIOSENSOR BY IMMOBILIZING MICROPEROXIDASE ON ANHDCT/GC ELECTRODE. JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY), 21(80 (CHEMISTRY ISSUE)), 117-127. SID. https://sid.ir/paper/70577/en

Vancouver: Copy

MADHI TAZEHKAND A., MOHAMMADI B., ZARGAZI M.H.. FABRICATION OF A NOVEL HYDROGEN PEROXIDE BIOSENSOR BY IMMOBILIZING MICROPEROXIDASE ON ANHDCT/GC ELECTRODE. JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY)[Internet]. 2011;21(80 (CHEMISTRY ISSUE)):117-127. Available from: https://sid.ir/paper/70577/en

IEEE: Copy

A. MADHI TAZEHKAND, B. MOHAMMADI, and M.H. ZARGAZI, “FABRICATION OF A NOVEL HYDROGEN PEROXIDE BIOSENSOR BY IMMOBILIZING MICROPEROXIDASE ON ANHDCT/GC ELECTRODE,” JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY), vol. 21, no. 80 (CHEMISTRY ISSUE), pp. 117–127, 2011, [Online]. Available: https://sid.ir/paper/70577/en

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