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Information Journal Paper

Title

Isolation and characterization of acid-soluble collagen from the skin of Rutilus Frisii Kutum (Kamensky) of the Caspian Sea

Pages

  768-779

Abstract

 This study proceeds to extract collagen from the skin of Rutilus Kutum, one of the main and economical species of the Caspian Sea. Acid-soluble collagen (ASC) from the skin of R. kutum was extracted and characterized by SDS-PAGE, FTIR and UV spectrophotometry. Based on the data from the SDS-PAGE and the composition of amino acid, it was probable that the obtained collagen classified as type I collagen. Collagen consists of two different types of α chains, including α 1 and α 2, and β components. Also, FTIR analysis revealed the presence of the helical arrangements of the collagen. Moreover, UV spectrophotometry exhibited this collagen has an absorbance at 220 nm. On the basis of dry weight, the yield of ASC was calculated as 15. 6%. The amount of proline in ASC was 89. 6 residues per 706. 1 residues, and 25. 7% of the collagen was composed of glycine. These results showed that R. Kutum skin could be considered as a possible source of collagen and can supply the theoretical basis for further research.

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  • Cite

    APA: Copy

    Naderi Garehgheshlagh, S., FATEMI, M.J., JAMILI, SH., SHARIFI, A.M., & NOURANI, M.R.. (2020). Isolation and characterization of acid-soluble collagen from the skin of Rutilus Frisii Kutum (Kamensky) of the Caspian Sea. IRANIAN JOURNAL OF FISHERIES SCIENCES, 19(2), 768-779. SID. https://sid.ir/paper/730811/en

    Vancouver: Copy

    Naderi Garehgheshlagh S., FATEMI M.J., JAMILI SH., SHARIFI A.M., NOURANI M.R.. Isolation and characterization of acid-soluble collagen from the skin of Rutilus Frisii Kutum (Kamensky) of the Caspian Sea. IRANIAN JOURNAL OF FISHERIES SCIENCES[Internet]. 2020;19(2):768-779. Available from: https://sid.ir/paper/730811/en

    IEEE: Copy

    S. Naderi Garehgheshlagh, M.J. FATEMI, SH. JAMILI, A.M. SHARIFI, and M.R. NOURANI, “Isolation and characterization of acid-soluble collagen from the skin of Rutilus Frisii Kutum (Kamensky) of the Caspian Sea,” IRANIAN JOURNAL OF FISHERIES SCIENCES, vol. 19, no. 2, pp. 768–779, 2020, [Online]. Available: https://sid.ir/paper/730811/en

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