High Performance Liquid Chromatographic Analysis of Reduction Products of a Thiolated DNA for Immobilization on Gold Nanoparticles

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JAVADI ZARNAGHI FATEMEH; Dastjerdi Khorzoghi Parisa

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Journal Paper

Paper Information

Journal: JOURNAL OF NANOSTRUCTURES Year:2019 | Volume:9 | Issue:3 Page(s): 429-436

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Title

High Performance Liquid Chromatographic Analysis of Reduction Products of a Thiolated DNA for Immobilization on Gold Nanoparticles

Author(s)

JAVADI ZARNAGHI FATEMEH | Dastjerdi Khorzoghi Parisa | Issue Writer Certificate

Keywords

DTT

Gold Nanoparticle

HPLC

Reduction

TCEP

Thiolated DNA

Abstract

DNA-based nano-biosensors are emerging scope in the field of biosensors. Many synthetic single stranded functional DNAs have been applied for development of such sensors, recently. Immobilization of DNA oligonucleotides on the surface of Gold Nanoparticles is a key step in the development of most colorimetric nano-biosensors. The bound DNA is usually thiolated and forms Au-S covalent bond to the surface of Gold Nanoparticles. To this endeavor, the DNA must get reduced prior to immobilization. There are a variety of approaches for Reduction of Thiolated DNAs that mostly employ Dithiothreitol (DTT). DTT-based DNA Reduction is not always complete and the sulfhydryl DNA (DSH) is not the sole product of the reaction. The results of the Reduction of a Thiolated DNA with DTT is analyzed in this paper with high performance liquid chromatography (HPLC) in order to find an optimal condition for DTT based Reduction. Finally, the optimal condition is compared to TCEPbased Reduction for the efficiency of DNA immobilization on the surface of Gold Nanoparticles.

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APA: Copy

JAVADI ZARNAGHI, FATEMEH, & Dastjerdi Khorzoghi, Parisa. (2019). High Performance Liquid Chromatographic Analysis of Reduction Products of a Thiolated DNA for Immobilization on Gold Nanoparticles. JOURNAL OF NANOSTRUCTURES, 9(3), 429-436. SID. https://sid.ir/paper/744920/en

Vancouver: Copy

JAVADI ZARNAGHI FATEMEH, Dastjerdi Khorzoghi Parisa. High Performance Liquid Chromatographic Analysis of Reduction Products of a Thiolated DNA for Immobilization on Gold Nanoparticles. JOURNAL OF NANOSTRUCTURES[Internet]. 2019;9(3):429-436. Available from: https://sid.ir/paper/744920/en

IEEE: Copy

FATEMEH JAVADI ZARNAGHI, and Parisa Dastjerdi Khorzoghi, “High Performance Liquid Chromatographic Analysis of Reduction Products of a Thiolated DNA for Immobilization on Gold Nanoparticles,” JOURNAL OF NANOSTRUCTURES, vol. 9, no. 3, pp. 429–436, 2019, [Online]. Available: https://sid.ir/paper/744920/en

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