THE INHERITANCE OF SSRS LOCI ALLELES IN OFFSPRING OF CROSSES BETWEEN GRAPES CVS. 'BIDANE QERMEZ' × 'MUSCAT HAMBURG'

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FATAHI MOGHADAM M.R.; ZAMANI Z.; EBADI A.; GHARAHYAZI BEHZAD; MEHLENBACHER S.A.

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Journal: IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY Year:2003 | Volume:3 | Issue:3-4 Page(s): 37-50

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Title

THE INHERITANCE OF SSRS LOCI ALLELES IN OFFSPRING OF CROSSES BETWEEN GRAPES CVS. 'BIDANE QERMEZ' × 'MUSCAT HAMBURG'

Author(s)

Keywords

ALLELES INHERITANCEQ4

FRUIT BREEDINGQ2

GRAPEQ4

MICROSATELLITE (SSRS)Q4

PARENTAGE STUDYQ4

Abstract

A population of offspring from crosses between 'Bidane Qermez' and 'Muscat Hamburg' GRAPE cultivars was used to investigate the inheritance of alleles at SSRs loci in controlled pollinated offspring. DNA was extracted from 46 seedlings as well as corresponding parents. Amplification of DNA was carried out using different colored fluorescent primers at 6 SSRs loci (VVS2, VVMD5, VVMD32, VVMD36, ssrZAG47 and ssrZAG79) in which the parents were completely polymorphic. Allele sizing was determined using ABI Prism 3100 DNA analyzer with Capillary Electrophoresis System at central laboratory of the Center for Gene Research at Oregon State University, U.S.A. Results showed that 94% of the seedlings shared one allele at each locus with parents indicating their hybrid nature, while 6% of the progenies with having only the female parent alleles were the result of self-pollination. Allele's distribution among the progenies determined their codominant inheritance. No significant differences (P>0.07) with Mendelian codominant ratio (1: 1: 1: 1) were observed using χ2 test. Three off springs resulted from self-pollination showed only the presence of maternal alleles either as homozygous or heterozygous. These results show the importance and potential of microsatellite loci in detecting parents-progeny relationship prior to study for inheritance of characters and alleles, bulked segregation analysis, gene and linkage maps and historical reconstruction of fruit trees pedigree.

Cites

PARENTAGE ANALYSIS OF SEEDLESS GRAPEVINE GENOTYPES WITH LIKELIHOOD BASED ASSIGNMENT APPROACHES

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APA: Copy

FATAHI MOGHADAM, M.R., ZAMANI, Z., EBADI, A., GHARAHYAZI, BEHZAD, & MEHLENBACHER, S.A.. (2003). THE INHERITANCE OF SSRS LOCI ALLELES IN OFFSPRING OF CROSSES BETWEEN GRAPES CVS. 'BIDANE QERMEZ' × 'MUSCAT HAMBURG'. IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, 3(3-4), 37-50. SID. https://sid.ir/paper/80727/en

Vancouver: Copy

FATAHI MOGHADAM M.R., ZAMANI Z., EBADI A., GHARAHYAZI BEHZAD, MEHLENBACHER S.A.. THE INHERITANCE OF SSRS LOCI ALLELES IN OFFSPRING OF CROSSES BETWEEN GRAPES CVS. 'BIDANE QERMEZ' × 'MUSCAT HAMBURG'. IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY[Internet]. 2003;3(3-4):37-50. Available from: https://sid.ir/paper/80727/en

IEEE: Copy

M.R. FATAHI MOGHADAM, Z. ZAMANI, A. EBADI, BEHZAD GHARAHYAZI, and S.A. MEHLENBACHER, “THE INHERITANCE OF SSRS LOCI ALLELES IN OFFSPRING OF CROSSES BETWEEN GRAPES CVS. 'BIDANE QERMEZ' × 'MUSCAT HAMBURG',” IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, vol. 3, no. 3-4, pp. 37–50, 2003, [Online]. Available: https://sid.ir/paper/80727/en

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