MICROPROPAGATION COMPARISON OF TWO CULTIVARS OF SWEET POTATO (IPOMOEA BATATAS L.) CULTURED IN THE SOUTH OF IRAN

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ASAD SANGABI K.; KHOUSHKHOUY M.

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Journal: IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY Year:2007 | Volume:7 | Issue:4 Page(s): 243-252

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Title

MICROPROPAGATION COMPARISON OF TWO CULTIVARS OF SWEET POTATO (IPOMOEA BATATAS L.) CULTURED IN THE SOUTH OF IRAN

Author(s)

ASAD SANGABI K. | KHOUSHKHOUY M. | Issue Writer Certificate

Keywords

IN VITRO CULTUREQ3

MICROPROPAGATIONQ3

SWEET POTATOQ3

TISSUE CULTUREQ2

Abstract

The suitable culture medium and hormonal requirements for TISSUE CULTURE of SWEET POTATO were studied. Tuberous roots of two cultivars namely 'White Type' and 'Red Type' were collected from Hormozgan province and cultured in the greenhouse of Department of Horticultural Science, College of Agriculture of Shiraz University. For disinfection, leaves and stem explants were immersed in 25% chlorox for 15 min and for tuberous roots, 60% chlorox for 30 minutes was used and then the explants were washed with sterilized distilled water. Explants were placed on MS culture medium containing concentrations of 0, 0.5, 1.0, 1.5, and 2.0 mg 1-1 NAA and 0, 0.5, 1.0, 1.5 and 2.0 mg 1-1 BA. The optimum concentration for callus regeneration was 1 mg 1-1 NAA with 2 mg 1-1 BA. The highest regeneration rates of root and shoot were obtained in the treatment of 1 mg 1-1 NAA and 0.5 mg 1-1 BA. Means root length, shoot length and shoot number were 40.50, 44.75 mm and 3.6 for stem explants and 34.50 mm and 2.20 for leaf explants, respectively, which were significantly different with other treatments. The second experiment was conducted for determination of the best hormonal concentrations of NAA and BA. In this experiment, MS medium containing concentrations of 0.0, 0.05, 0.1, 0.2, and 0.5mg 1-1 BA and a constant level of 1 mg 1-1 NAA was used. The highest root and shoot in the second experiment were obtained with 0.1 mg 1-1BA and 1mg 1-1 NAA. In this treatment, the means of root length shoot length and the numbers of shoots were 85.50, 68.75 mm and 6 for stem explants, respectively, the differences were significant in both cases. The shoot formed on the plants kept on the culture medium for about 100 days. To compare the 'White Type' and 'Red Type' cultivars, stem explants of both cultivars were cultured on the media containing 0, 0.05, 0.1, 0.2, and 0.5 mg 1-1BA and 1 mg 1-1 NAA. The highest numbers of root and stem in both cultivars were obtained with 0.1 mg 1-1 BA and 1.0 mg 1-1 NAA, which were statistically significant compared to other treatments. Shoot regeneration rate in the 'White Type' (6) was significantly more than that of the 'Red Type' (3.6). Regeneration capacities of the 'White Type' and 'Red Type' in all explants were increased when the light intensity of 50mmol m-2 S-1 was used.

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APA: Copy

ASAD SANGABI, K., & KHOUSHKHOUY, M.. (2007). MICROPROPAGATION COMPARISON OF TWO CULTIVARS OF SWEET POTATO (IPOMOEA BATATAS L.) CULTURED IN THE SOUTH OF IRAN. IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, 7(4), 243-252. SID. https://sid.ir/paper/80735/en

Vancouver: Copy

ASAD SANGABI K., KHOUSHKHOUY M.. MICROPROPAGATION COMPARISON OF TWO CULTIVARS OF SWEET POTATO (IPOMOEA BATATAS L.) CULTURED IN THE SOUTH OF IRAN. IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY[Internet]. 2007;7(4):243-252. Available from: https://sid.ir/paper/80735/en

IEEE: Copy

K. ASAD SANGABI, and M. KHOUSHKHOUY, “MICROPROPAGATION COMPARISON OF TWO CULTIVARS OF SWEET POTATO (IPOMOEA BATATAS L.) CULTURED IN THE SOUTH OF IRAN,” IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, vol. 7, no. 4, pp. 243–252, 2007, [Online]. Available: https://sid.ir/paper/80735/en

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