IN VITRO CULTURE OF HERBACEOUS PEONY (PAEONIA LACTIFLORA ANDR)

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ESLAHI F.; KHOUSHKHOUY M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY Year:2003 | Volume:4 | Issue:1-2 Page(s): 43-50

Download Full-Text

Persian Verion

View:

1,324

Download:

Cites:

Information Journal Paper

Title

IN VITRO CULTURE OF HERBACEOUS PEONY (PAEONIA LACTIFLORA ANDR)

Author(s)

ESLAHI F. | KHOUSHKHOUY M. | Issue Writer Certificate

Keywords

CALLUS FORMATION. HERBACEOUS PEONYQ3

IN VITRO CULTURE. SHOOT PROLIFERATIONQ3

Abstract

Peonies are commonly propagated by dividing the roots or rhizomes but a few number of plants are obtained by these methods. Peonies can also be propagated by seeds but it is not usually practiced due to having epicotyl dormancy and long (3 to 7 yr) juvenility stage. Consequently, a quick micropropagation method would overcome these problems and could also be used for the multiplication of virus-free stock materials or introducing new cultivars. Present investigation was conducted during 2000 to 2002 to find the best medium for herbaceous peony in in vitro culture. Herbaceous peony plants were purchased from Karaj city and transferred to the greenhouse of the Department of Horticultural Science, College of Agriculture, Shiraz University, Shiraz, Iran. Explants of different parts of peony (internode, stem, leaf, petiole and rhizome) were examined for micropropagation studies. After surface sterilization, for shoot proliferation, the internode explants were placed on Murashing and Skoog (MS) medium salts plus 20 g r1 sucrose and 8 g I-1 agar supplemented with 0 to 2 mg I-1 6-benzyladenine (BA) and kinetin (Kin). To improve the multiplication rate, 0.1 mg-1 gibberellic acid (GA3) and indole-3-butyric acid (IBA) were also used in the medium The explants were placed under cool-white fluorescent tubes with 1.5 k lux light in a day length of 16 hr and 25°C temperature. For root formation, the explants were placed on a half-strength MS medium supplemented with 0 to 2 mg I-1 indole-3-butyric acid (IBA) as well as 0 to I mg I-1 I-naphthaleneacetic acid (NAA) or 2A-dichlorophenoxyacetic acid (2.4-D) and kept under dark or light conditions. For callus formation, the explants were placed on MS medium supplemented with a combination of 0 to 1 mg I-1 2.4-0, BA and NAA. The best treatment for shoot proliferation of nodal stems was MS medium supplemented with 1.4 mg I-1 BA and 0.1 mg I-1 GA3 Best treatment for shoot proliferation was using 1.4 mg I-1 BA and 0.1 mg r1 GA3. All stem explants produced callus on MS medium supplemented with 0.5 mg I-1 BA, 0.5 mg I-1 NAA and 0.1 mg I-1 2A-0. All stem explants produced callus on MS medium supplemented with 0.5 mg I-1 BA. 0.5 mg I-1 NAA and 0.1 mg I-1 2.4More studies are needed to establish root formation in herbaceous peony.

Cites

No record.

References

No record.

Cite

APA: Copy

ESLAHI, F., & KHOUSHKHOUY, M.. (2003). IN VITRO CULTURE OF HERBACEOUS PEONY (PAEONIA LACTIFLORA ANDR). IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, 4(1-2), 43-50. SID. https://sid.ir/paper/80800/en

Vancouver: Copy

ESLAHI F., KHOUSHKHOUY M.. IN VITRO CULTURE OF HERBACEOUS PEONY (PAEONIA LACTIFLORA ANDR). IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY[Internet]. 2003;4(1-2):43-50. Available from: https://sid.ir/paper/80800/en

IEEE: Copy

F. ESLAHI, and M. KHOUSHKHOUY, “IN VITRO CULTURE OF HERBACEOUS PEONY (PAEONIA LACTIFLORA ANDR),” IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, vol. 4, no. 1-2, pp. 43–50, 2003, [Online]. Available: https://sid.ir/paper/80800/en

Related Journal Papers