STABILITY OF RECOMBINANT PROTEINS IN ESCHERICHIA COLI: THE EFFECT OF CO-EXPRESSION OF FIVE DIFFERENT CHAPERONE SETS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MIRZAHOSSEINI H.; ALIBOLANDI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Sciences, Islamic Republic of Iran Year:2009 | Volume:20 | Issue:4 Page(s): 305-310

Download Full-Text

Persian Verion

View:

1,416

Download:

215

Cites:

Information Journal Paper

Title

STABILITY OF RECOMBINANT PROTEINS IN ESCHERICHIA COLI: THE EFFECT OF CO-EXPRESSION OF FIVE DIFFERENT CHAPERONE SETS

Author(s)

MIRZAHOSSEINI H. | ALIBOLANDI M. | Issue Writer Certificate

Keywords

CHAPERONES COMBINATIONQ3

RECOMBINANT PROTEIN STABILITYQ3

HBFGFQ3

Abstract

Chaperones are produced by prokaryotic, yeast and higher eukaryotic cells for various purposes. Over-expression of each chaperone or sets of them affect the production level of a recombinant protein in the cell. On the basis of this hypothesis, five different plasmids with 5 different combinations of 6 chaperones molecule, transformed into Escherichia coli along with human basic Fibroblast Growth Factor expression plasmid. Each transformant that contain both plasmids for expression of HBFGF and chaperone combinations was induced with proper concentration of related inducers. Subsequently, total amount of produced HBFGF was analyzed based on SDS-PAGE and ELISA. Our results indicated that “TF” and “DnaK/DnaJ/GrpE” destabilized HBFGF, while “DnaK/DnaJ/GrpE/GroEL/GroES” and “GroEL/GroES” combinations were able to stabilize it. It has also revealed that “GroEL/GroES/TF” combination negatively affected the HBFGF production.

Cites

No record.

References

No record.

Cite

APA: Copy

MIRZAHOSSEINI, H., & ALIBOLANDI, M.. (2009). STABILITY OF RECOMBINANT PROTEINS IN ESCHERICHIA COLI: THE EFFECT OF CO-EXPRESSION OF FIVE DIFFERENT CHAPERONE SETS. JOURNAL OF SCIENCES ISLAMIC REPUBLIC OF IRAN, 20(4), 305-310. SID. https://sid.ir/paper/84143/en

Vancouver: Copy

MIRZAHOSSEINI H., ALIBOLANDI M.. STABILITY OF RECOMBINANT PROTEINS IN ESCHERICHIA COLI: THE EFFECT OF CO-EXPRESSION OF FIVE DIFFERENT CHAPERONE SETS. JOURNAL OF SCIENCES ISLAMIC REPUBLIC OF IRAN[Internet]. 2009;20(4):305-310. Available from: https://sid.ir/paper/84143/en

IEEE: Copy

H. MIRZAHOSSEINI, and M. ALIBOLANDI, “STABILITY OF RECOMBINANT PROTEINS IN ESCHERICHIA COLI: THE EFFECT OF CO-EXPRESSION OF FIVE DIFFERENT CHAPERONE SETS,” JOURNAL OF SCIENCES ISLAMIC REPUBLIC OF IRAN, vol. 20, no. 4, pp. 305–310, 2009, [Online]. Available: https://sid.ir/paper/84143/en

Related Journal Papers