DETERMINATION OF RT-PCR DETECTION LIMIT OF LIVE AND DEAD SALMONELLA CELLS IN RAW AND STERILIZED MILK (SHORT PAPER)

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HOSSEINZADEH S.; SHEKARFOROUSH S.S.; FAZELI M.; DEHGHAN M.

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Paper Information

Journal: IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR) Year:2014 | Volume:15 | Issue:1 (46) Page(s): 58-61

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Information Journal Paper

Title

DETERMINATION OF RT-PCR DETECTION LIMIT OF LIVE AND DEAD SALMONELLA CELLS IN RAW AND STERILIZED MILK (SHORT PAPER)

Author(s)

HOSSEINZADEH S. | SHEKARFOROUSH S.S. | FAZELI M. | DEHGHAN M. | Issue Writer Certificate

Keywords

SALMONELLAQ2

REVERSE TRANSCRIPTASE PCRQ2

MILKQ2

Abstract

The objective of the current study was to evaluate the reproducibility of a REVERSE TRANSCRIPTASE PCR (RT-PCR) -based technique to differentiate viable and dead SALMONELLA cells in raw and sterilized MILK. The microorganism was initially inoculated into the MILK samples followed by incubating at 37°C for 4 h prior to inactivation by heat at 80oC for 10 min. The treated and non-treated samples were subsequently monitored using both PCR and RT-PCR, in vitro. Following 4 h incubation, the invA gene of SALMONELLA was clearly amplified by RT-PCR, while no band was detected in the heated samples. On the other hand, using the conventional PCR, it was possible to amplify the gene in both samples. Our results may suggest an important application of the RT-PCR technique, especially when the number of live organisms is an imperative factor to produce food borne infections and to establish a detection limit of the test.

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APA: Copy

HOSSEINZADEH, S., SHEKARFOROUSH, S.S., FAZELI, M., & DEHGHAN, M.. (2014). DETERMINATION OF RT-PCR DETECTION LIMIT OF LIVE AND DEAD SALMONELLA CELLS IN RAW AND STERILIZED MILK (SHORT PAPER). IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), 15(1 (46)), 58-61. SID. https://sid.ir/paper/85638/en

Vancouver: Copy

HOSSEINZADEH S., SHEKARFOROUSH S.S., FAZELI M., DEHGHAN M.. DETERMINATION OF RT-PCR DETECTION LIMIT OF LIVE AND DEAD SALMONELLA CELLS IN RAW AND STERILIZED MILK (SHORT PAPER). IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR)[Internet]. 2014;15(1 (46)):58-61. Available from: https://sid.ir/paper/85638/en

IEEE: Copy

S. HOSSEINZADEH, S.S. SHEKARFOROUSH, M. FAZELI, and M. DEHGHAN, “DETERMINATION OF RT-PCR DETECTION LIMIT OF LIVE AND DEAD SALMONELLA CELLS IN RAW AND STERILIZED MILK (SHORT PAPER),” IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), vol. 15, no. 1 (46), pp. 58–61, 2014, [Online]. Available: https://sid.ir/paper/85638/en

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