REPLACEMENT OF SALAMON WITH SHOTOR DILUENT AND EGG YOLK WITH LOW DENSITY LIPOPROTEIN FOR CHILLED STORAGE OF RAM SEMEN

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GHARIBI S.; NIASARI NASLAJI A.; POURSASAN N.; MOOSAVI MOVAHEDI A.A.

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Journal: IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR) Year:2014 | Volume:15 | Issue:3 (48) Page(s): 279-284

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Title

REPLACEMENT OF SALAMON WITH SHOTOR DILUENT AND EGG YOLK WITH LOW DENSITY LIPOPROTEIN FOR CHILLED STORAGE OF RAM SEMEN

Author(s)

GHARIBI S. | NIASARI NASLAJI A. | POURSASAN N. | MOOSAVI MOVAHEDI A.A. | Issue Writer Certificate

Keywords

RAMQ1

SEMEN PRESERVATIONQ2

LOW DENSITY LIPOPROTEINQ2

DILUENTQ1

Abstract

Summary: The present study investigated the possibility of replacing salamon with modified shotor DILUENT (MSD) and egg yolk (EY) with LOW DENSITY LIPOPROTEIN (LDL) for chilled storage of RAM semen. Good quality semen (>80% progressive forward motility (PFM) of sperm) from 3 fertile RAMs was collected using an artificial vagina and pooled for each experiment. LOW DENSITY LIPOPROTEIN was extracted from fresh EY. In experiment 1, semen was divided into 2 fractions and extended in MSD or salamon. In experiment 2, semen was assigned into 5 fractions and extended in MSD supplemented with 12 and 15% EY or 3, 5 and 8% LDL. In experiment 3, semen was divided into 2 fractions and extended in MSD supplemented with 12% EY or 5% LDL. Viability of sperm was assessed at times 0 (immediately after semen dilution), 2 or 4 (at 4oC) and up to 72 h after semen dilution. Data was analyzed using General Linear Model (GLM) procedure, including repeated measures. In experiment 1, the viability of sperm was similar in two DILUENTs (P>0.05). In experiment 2, PFM of sperm was similar among groups at the time of dilution (P>0.05); but remained elevated in 5 and 8% LDL compared to other groups afterward (P<0.05). In experiment 3, PFM of sperm was superior at 48 and 72 h after dilution in 5% LDL compared to 12% EY (P<0.05). In conclusion, MSD supplemented with 5% LDL is a suitable DILUENT for RAM fresh semen preserved at 4oC for 72 h.

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APA: Copy

GHARIBI, S., NIASARI NASLAJI, A., POURSASAN, N., & MOOSAVI MOVAHEDI, A.A.. (2014). REPLACEMENT OF SALAMON WITH SHOTOR DILUENT AND EGG YOLK WITH LOW DENSITY LIPOPROTEIN FOR CHILLED STORAGE OF RAM SEMEN. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), 15(3 (48)), 279-284. SID. https://sid.ir/paper/85688/en

Vancouver: Copy

GHARIBI S., NIASARI NASLAJI A., POURSASAN N., MOOSAVI MOVAHEDI A.A.. REPLACEMENT OF SALAMON WITH SHOTOR DILUENT AND EGG YOLK WITH LOW DENSITY LIPOPROTEIN FOR CHILLED STORAGE OF RAM SEMEN. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR)[Internet]. 2014;15(3 (48)):279-284. Available from: https://sid.ir/paper/85688/en

IEEE: Copy

S. GHARIBI, A. NIASARI NASLAJI, N. POURSASAN, and A.A. MOOSAVI MOVAHEDI, “REPLACEMENT OF SALAMON WITH SHOTOR DILUENT AND EGG YOLK WITH LOW DENSITY LIPOPROTEIN FOR CHILLED STORAGE OF RAM SEMEN,” IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), vol. 15, no. 3 (48), pp. 279–284, 2014, [Online]. Available: https://sid.ir/paper/85688/en

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