TITRATION OF THE IRANIAN WHITE SPOT VIRUS ISOLATE, ON CRAYFISH ASTACUS LEPTODACTYLUS AND PENAEUS SEMISULCATUS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOTAMEDI SEDEH F.; AFSHARNASAB M.; HEIDAREH M.; SHAFAEE S.K.; RAJABIFAR S.; DASHTIANNASAB A.; RAZAVI M.H.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Fisheries Sciences Year:2012 | Volume:11 | Issue:1 Page(s): 145-155

Download Full-Text

Persian Verion

View:

1,990

Download:

188

Cites:

Information Journal Paper

Title

TITRATION OF THE IRANIAN WHITE SPOT VIRUS ISOLATE, ON CRAYFISH ASTACUS LEPTODACTYLUS AND PENAEUS SEMISULCATUS

Author(s)

Keywords

WHITE SPOT VIRUSQ3

TITRATIONQ3

KARBER FORMULAQ3

ASTACUS LEPTODACTYLUSQ3

PENAEUS SEMISULCATUSQ3

Abstract

WHITE SPOT VIRUS (WSV) is currently the most serious viral pathogen of shrimp worldwide; it causes mortality up to 100% within 7-10 days in commercial shrimp farms. Infected Indian white shrimp Fenneropenaeus indicus samples were collected from Guatr shrimp site in Sistan and Baluchestan province in south of Iran and WSV infection was confirmed by Nested PCR. WSV was isolated from infected shrimp samples by centrifugation and filtration and multiplied in crayfish by intramuscular inoculation, the isolated virus was called WSV/IRN/1/2010. In order to determine the dilution resulting in 90-100% mortality in Penaeus semiculcatus, diluted virus stock in steps from 100 till 105 times in sterile PBS was injected intramuscularly to 14 shrimps in each group. Also the virus stock was diluted in steps from 1/2 till 1/32 times in sterile PBS and injected intramuscularly in ASTACUS LEPTODACTYLUS crayfish. Therefore the LD50 of live virus stock in ASTACUS LEPTODACTYLUS and Penaeus semiculcatus crayfish were calculated by the Karber method 103.29 /ml and 105.35 /ml, respectively.

Cites

No record.

References

No record.

Cite

APA: Copy

MOTAMEDI SEDEH, F., AFSHARNASAB, M., HEIDAREH, M., SHAFAEE, S.K., RAJABIFAR, S., DASHTIANNASAB, A., & RAZAVI, M.H.. (2012). TITRATION OF THE IRANIAN WHITE SPOT VIRUS ISOLATE, ON CRAYFISH ASTACUS LEPTODACTYLUS AND PENAEUS SEMISULCATUS. IRANIAN JOURNAL OF FISHERIES SCIENCES, 11(1), 145-155. SID. https://sid.ir/paper/86054/en

Vancouver: Copy

MOTAMEDI SEDEH F., AFSHARNASAB M., HEIDAREH M., SHAFAEE S.K., RAJABIFAR S., DASHTIANNASAB A., RAZAVI M.H.. TITRATION OF THE IRANIAN WHITE SPOT VIRUS ISOLATE, ON CRAYFISH ASTACUS LEPTODACTYLUS AND PENAEUS SEMISULCATUS. IRANIAN JOURNAL OF FISHERIES SCIENCES[Internet]. 2012;11(1):145-155. Available from: https://sid.ir/paper/86054/en

IEEE: Copy

F. MOTAMEDI SEDEH, M. AFSHARNASAB, M. HEIDAREH, S.K. SHAFAEE, S. RAJABIFAR, A. DASHTIANNASAB, and M.H. RAZAVI, “TITRATION OF THE IRANIAN WHITE SPOT VIRUS ISOLATE, ON CRAYFISH ASTACUS LEPTODACTYLUS AND PENAEUS SEMISULCATUS,” IRANIAN JOURNAL OF FISHERIES SCIENCES, vol. 11, no. 1, pp. 145–155, 2012, [Online]. Available: https://sid.ir/paper/86054/en

Related Journal Papers