DETERMINATION OF SUITABLE HORMONAL COMBINATION AND CONCENTRATION FOR IN VITRO CULTURE OF MATURE GINKGO BILOBA EXPLANTS

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GHAEM MAGHAMI S.A.; LABAFI Y.A.

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Journal: Journal of Agricultural Sciences and Natural Resources Year:2005 | Volume:12 | Issue:1 Page(s): 137-146

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Title

DETERMINATION OF SUITABLE HORMONAL COMBINATION AND CONCENTRATION FOR IN VITRO CULTURE OF MATURE GINKGO BILOBA EXPLANTS

Author(s)

GHAEM MAGHAMI S.A. | LABAFI Y.A. | Issue Writer Certificate

Keywords

GINKGO BILOBAQ4

IN VITRO CULTUREQ3

MATURE TREEQ4

PLANT GROWTH REGULATORQ4

Abstract

GINKGO BILOBA one of the primary gymnosperms is important in term of ornamental and medicinal usage because of its Ginkgolide and Flavonoid contents. Earlier research embryo has tended to emphasize specific use of embryo to obtain explants. Three experiments was conducted to determine the kinde of hormones and their proper concentrations on growth potential of mature GINKGO BILOBA explants (single node). In first experiment, a combination of two hormones of BA (0.0, 0.05, 5.4, 16.1 µM) and NAA (0.0, 0.05, 5.4 16.1, 26.1, 37.6 µM), were cultured on Murashige and Skoog medium. The result showed that the 0.05 µM concentration could increase the explants growth in both hormones. In second experiment, different hormones (IAA, IBA, NAA, 2, 4-D, BA, 2ip, BPA, Zeatin) separately or as combination with obtained proper concentration in first experiment (0.05 µM) was used. The results showed that two hormones of Zeatin and IBA had best results on growth of explants. Third experiment was conducted to find the best concentration of Zeatin (0.01, 0.05, 0.25, 1.25. 2.5 µM) and IBA (0.002,0.01,0.05,0.25 µM)obtained hormones in second experiment. It was concluded that Zeatin and IBA with 0.25 and 0.01 µM had proper growth of shoots and leaves respectively.

Cites

PROPAGATION OF GINKGO BILOBA L. THROUGH TISSUE CULTURE OF VARIOUS PLANT PARTS

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APA: Copy

GHAEM MAGHAMI, S.A., & LABAFI, Y.A.. (2005). DETERMINATION OF SUITABLE HORMONAL COMBINATION AND CONCENTRATION FOR IN VITRO CULTURE OF MATURE GINKGO BILOBA EXPLANTS. JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES, 12(1), 137-146. SID. https://sid.ir/paper/9090/en

Vancouver: Copy

GHAEM MAGHAMI S.A., LABAFI Y.A.. DETERMINATION OF SUITABLE HORMONAL COMBINATION AND CONCENTRATION FOR IN VITRO CULTURE OF MATURE GINKGO BILOBA EXPLANTS. JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES[Internet]. 2005;12(1):137-146. Available from: https://sid.ir/paper/9090/en

IEEE: Copy

S.A. GHAEM MAGHAMI, and Y.A. LABAFI, “DETERMINATION OF SUITABLE HORMONAL COMBINATION AND CONCENTRATION FOR IN VITRO CULTURE OF MATURE GINKGO BILOBA EXPLANTS,” JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES, vol. 12, no. 1, pp. 137–146, 2005, [Online]. Available: https://sid.ir/paper/9090/en

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