ISOLATION OF DNA APTAMER AGAINST A.BAUMANNII BY COUNTER SELEX ASSAY

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RASOULINEJAD SAMANEH; MOUSAVI GARGARI SEYED LATIF

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Seminar Paper

Paper Information

Seminar: INTERNATIONAL AND IRANIAN CONGRESS OF MICROBIOLOGY
Year:2015 | Issue:16

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Title

ISOLATION OF DNA APTAMER AGAINST A.BAUMANNII BY COUNTER SELEX ASSAY

Author(s)

RASOULINEJAD SAMANEH | MOUSAVI GARGARI SEYED LATIF | Issue Writer Certificate

Keywords

GENE LIBRARY

DNA SINGLE-STRANDED

OSTEOMYELITIS

DISEASE OUTBREAKS

FLOW CYTOMETRY

Abstract

BACKGROUND AND AIM: ACINETOBACTER BAUMANNII IS A UBIQUITOUS PATHOGEN WITH PROPENSITY TO CAUSE LARGE, OFTEN MULTIFACILITY, NOSOCOMIAL OUTBREAKS AND CAUSE MENINGITIS, OSTEOMYELITIS, PNEUMONIA, SEPTICEMIA THAT PARTICULARLY AFFECTS PATIENTS IN THE INTENSIVE CARE AND BURN UNIT. IT HAS BECOME A SERIOUS CHALLENGE TO NEW HEALTHCARE SYSTEMS WITH STRONG CAPACITY TO DEVELOP ANTIMICROBIAL RESISTANCE. QUICK AND ACCURATE DETECTION IS CHALLENGING IN MANY MICROBIAL DIAGNOSTIC APPROACHES. DNA APTAMERS ARE ALTERNATIVE LIGANDS TO CAPTURE PATHOGENS. THEY HAVE LOTS OF ADVANTAGES OVER ANTIBODIES SUCH AS, HIGH TARGET SPECIFICITY, LOWER COST & IMMUNOGENICITY, SMALL SIZE, EASE OF SYNTHESIS AND LABELING. ISOLATED APTAMER SEQUENCE ARE GENERATED THROUGH THE SYSTEMATIC EVOLUTION OF LIGANDS BY EXPONENTIAL ENRICHMENT (SELEX) PROCESS, A TECHNIQUE USED FOR THE SCREENING OF LARGE LIBRARIES OF OLIGONUCLEOTIDES BY AN ITERATIVE PROCESS OF IN VITRO SELECTION AND AMPLIFICATION…..

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APA: Copy

RASOULINEJAD, SAMANEH, & MOUSAVI GARGARI, SEYED LATIF. (2015). ISOLATION OF DNA APTAMER AGAINST A.BAUMANNII BY COUNTER SELEX ASSAY. INTERNATIONAL AND IRANIAN CONGRESS OF MICROBIOLOGY. SID. https://sid.ir/paper/940099/en

Vancouver: Copy

RASOULINEJAD SAMANEH, MOUSAVI GARGARI SEYED LATIF. ISOLATION OF DNA APTAMER AGAINST A.BAUMANNII BY COUNTER SELEX ASSAY. 2015. Available from: https://sid.ir/paper/940099/en

IEEE: Copy

SAMANEH RASOULINEJAD, and SEYED LATIF MOUSAVI GARGARI, “ISOLATION OF DNA APTAMER AGAINST A.BAUMANNII BY COUNTER SELEX ASSAY,” presented at the INTERNATIONAL AND IRANIAN CONGRESS OF MICROBIOLOGY. 2015, [Online]. Available: https://sid.ir/paper/940099/en

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