Stimulation of Cardiomyocyte by Light

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Keshmiri Neghab Hoda; SABOURY ALI AKBAR; ESMAEELI DJAVID GHOLAMREZA; PORNOUR MAJID; Hong Jun; GOLIAEI BAHRAM

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Seminar: CONGRESS OF WOUND AND TISSUE REPAIR
Year:2019 | Issue:6

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Title

Stimulation of Cardiomyocyte by Light

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Abstract

Background: Electrical stimulation is the standard technique for exploring electrical behavior of heart muscle, but this approach has considerable technical limitations. Here we report expression of the light-activated cation channel channelrhodopsin-2 for light-induced stimulation of heart muscle in vitro. Methods: For this purpose, primary cardiomyocytes (CM) were isolated and lentiviral delivery of ChR2 (H134R) in cardiomyocytes was done by infection. A stable channelrhodopsin-2 (ChR2) expressing cardiomyocyte was developed, characterized and used. Optical imaging of voltage or calcium was performed using voltage-sensitive dye Di-8-ANEEPPS, and intracellular calcium was imaged with Fura2-AM. Results: Our result demonstrated that following blue-light illumination CM-ChR2 exhibited 26% (P < 0. 05) enhancement of membrane potential as a result of depolarization compared with non-stimulated samples and 43% elevation of intracellular calcium compared with the non-stimulated group was observed. Conclusion: This method enabled precise localized stimulation and constant prolonged depolarization of cardiomyocytes resulting in alterations of membrane potential and Ca2+ homeostasis.

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APA: Copy

Keshmiri Neghab, Hoda, SABOURY, ALI AKBAR, ESMAEELI DJAVID, GHOLAMREZA, PORNOUR, MAJID, Hong, Jun, & GOLIAEI, BAHRAM. (2019). Stimulation of Cardiomyocyte by Light. 4ST INTERNATIONAL AND 6RD NATIONAL CONGRESS OF WOUND AND TISSUE REPAIR (the future of wounds and sca. SID. https://sid.ir/paper/949190/en

Vancouver: Copy

Keshmiri Neghab Hoda, SABOURY ALI AKBAR, ESMAEELI DJAVID GHOLAMREZA, PORNOUR MAJID, Hong Jun, GOLIAEI BAHRAM. Stimulation of Cardiomyocyte by Light. 2019. Available from: https://sid.ir/paper/949190/en

IEEE: Copy

Hoda Keshmiri Neghab, ALI AKBAR SABOURY, GHOLAMREZA ESMAEELI DJAVID, MAJID PORNOUR, Jun Hong, and BAHRAM GOLIAEI, “Stimulation of Cardiomyocyte by Light,” presented at the 4ST INTERNATIONAL AND 6RD NATIONAL CONGRESS OF WOUND AND TISSUE REPAIR (the future of wounds and sca. 2019, [Online]. Available: https://sid.ir/paper/949190/en

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