STUDY OF POLYMORPHISM IN INTRON 4 AND EXON 5 OF GHRELIN GENE IN SOME MASSES OF KHUZESTAN NATIVE CHICKENS USING PCR-RFLP

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REZAEI YAZD ABADI S.; ROSHANFEKR H.; BEIGI NASIRI M.T.; FAYAZI J.

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Paper Information

Journal: SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL Year:2017 | Volume:13 | Issue:2 Page(s): 22-28

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Title

STUDY OF POLYMORPHISM IN INTRON 4 AND EXON 5 OF GHRELIN GENE IN SOME MASSES OF KHUZESTAN NATIVE CHICKENS USING PCR-RFLP

Author(s)

REZAEI YAZD ABADI S. | ROSHANFEKR H. | BEIGI NASIRI M.T. | FAYAZI J. | Issue Writer Certificate

Keywords

KHOUZESTAN NATIVE CHICKENSQ1

GHRELIN GENEQ1

PCR-RFLPQ2

Abstract

Ghrelin is a stimulating peptide for growth hormone in poultry, which is composed of 26 amino acids. With the release of growth hormone, ghrelin stimulates food intake and body weight gain leads to energy balance. This study was performed in order to identify polymorphism in fourth intron and fifth exon in Khuzestan province native chicken's GHRELIN GENE using the PCR-RFLP method. Therefore, random blood sampling from 100 chickens of native chickens in Khuzestan province breeding and Jihad livestock inputs was performed. DNA extraction from blood samples was performed using the salt method. The quantity and quality of the extracted DNA were determined using gel electrophoresis (1%) and nanodrop. Then, 228 bp fragment of fourth intron and fifth exon region were amplified using polymerase chain reaction (PCR). Finally, the amplified samples were digested with HaeIII enzyme, and the digestion products of 173 and 55 bp were found. The results showed that in all samples after enzymatic digestion there was only one band form. Therefore, with respect to the similarity of studied genotypes in this study, mutations were not verified. The absent of polymorphism in the studied mass (population) could be due to the environmental conditions and relatively low number of samples.

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APA: Copy

REZAEI YAZD ABADI, S., ROSHANFEKR, H., BEIGI NASIRI, M.T., & FAYAZI, J.. (2017). STUDY OF POLYMORPHISM IN INTRON 4 AND EXON 5 OF GHRELIN GENE IN SOME MASSES OF KHUZESTAN NATIVE CHICKENS USING PCR-RFLP. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, 13(2), 22-28. SID. https://sid.ir/paper/97081/en

Vancouver: Copy

REZAEI YAZD ABADI S., ROSHANFEKR H., BEIGI NASIRI M.T., FAYAZI J.. STUDY OF POLYMORPHISM IN INTRON 4 AND EXON 5 OF GHRELIN GENE IN SOME MASSES OF KHUZESTAN NATIVE CHICKENS USING PCR-RFLP. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL[Internet]. 2017;13(2):22-28. Available from: https://sid.ir/paper/97081/en

IEEE: Copy

S. REZAEI YAZD ABADI, H. ROSHANFEKR, M.T. BEIGI NASIRI, and J. FAYAZI, “STUDY OF POLYMORPHISM IN INTRON 4 AND EXON 5 OF GHRELIN GENE IN SOME MASSES OF KHUZESTAN NATIVE CHICKENS USING PCR-RFLP,” SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, vol. 13, no. 2, pp. 22–28, 2017, [Online]. Available: https://sid.ir/paper/97081/en

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