COMPARISON OF THE CYTOPLASMIC PROTEINS OF FUSARIUM SOLANI ISOLATE, OBTAINED FROM AIR AND FOOD SOURCES IN IRAN BY SDS - PAGE

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FATAHINIA MAHNAZ; KHOSRAVI A.R.; YADGARI M.H.

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Journal: SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL Year:2009 | Volume:4 | Issue:5 (22) Page(s): 42-48

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Title

COMPARISON OF THE CYTOPLASMIC PROTEINS OF FUSARIUM SOLANI ISOLATE, OBTAINED FROM AIR AND FOOD SOURCES IN IRAN BY SDS - PAGE

Author(s)

FATAHINIA MAHNAZ | KHOSRAVI A.R. | YADGARI M.H. | Issue Writer Certificate

Keywords

FUSARIUM SOLANIQ3

ANTIGENQ3

SDS-PAGEQ3

Abstract

Studies have shown that various species of Fusarium grow well in food resources, specially in agricultural crops like cotton meal, rough rice, potato and wheat. Also by distributing conidia in air and inhaling them, they can act as pathogen, saprophyte or allergenic agents. FUSARIUM SOLANI is known as soil saprophytic and it has allergenic components in cell wall and cytoplasm of conidia and hyphae, used for diagnostic and allergy therapy purposes. The goal of this study was to identify the cytoplasmic proteins of FUSARIUM SOLANI isolates under study and evaluate the correlation and differention of the ANTIGENs between isolates. In the present study, 12 samples of FUSARIUM SOLANI, with sources of air and foods, were maintained at collection of mycology center of faculty of Veterinary Medicine of Tehran University. They were broken by Freez and Taw and mechanical methods, and after centrifuging, supernatants which consist of most cytoplasmic proteins were assessed by SDS-PAGE technique.The results showed that 12 FUSARIUM SOLANI isolates have common and uncommon protein bands and some isolates have special protein bands such as 87, 40 and 108 KD, in which specifics for isolates were 3, 4 and 10 respectively. Also, the bands of 24 and 32 KD were presented on the all isolates. In comparison with two standard strains, UAMH 7419 and UAMH 3317, the 24 KD band is specific for Iranian isolates, but 32 KD band was presented in standard strains. Therefore, the 24 KD band is special protein in Iran's native samples. This study suggests that for diagnostic and vaccine preparation and allergy therapy, the specific native FUSARIUM SOLANI ANTIGENs should be used by researchers.

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APA: Copy

FATAHINIA, MAHNAZ, KHOSRAVI, A.R., & YADGARI, M.H.. (2009). COMPARISON OF THE CYTOPLASMIC PROTEINS OF FUSARIUM SOLANI ISOLATE, OBTAINED FROM AIR AND FOOD SOURCES IN IRAN BY SDS - PAGE. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, 4(5 (22)), 42-48. SID. https://sid.ir/paper/97446/en

Vancouver: Copy

FATAHINIA MAHNAZ, KHOSRAVI A.R., YADGARI M.H.. COMPARISON OF THE CYTOPLASMIC PROTEINS OF FUSARIUM SOLANI ISOLATE, OBTAINED FROM AIR AND FOOD SOURCES IN IRAN BY SDS - PAGE. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL[Internet]. 2009;4(5 (22)):42-48. Available from: https://sid.ir/paper/97446/en

IEEE: Copy

MAHNAZ FATAHINIA, A.R. KHOSRAVI, and M.H. YADGARI, “COMPARISON OF THE CYTOPLASMIC PROTEINS OF FUSARIUM SOLANI ISOLATE, OBTAINED FROM AIR AND FOOD SOURCES IN IRAN BY SDS - PAGE,” SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, vol. 4, no. 5 (22), pp. 42–48, 2009, [Online]. Available: https://sid.ir/paper/97446/en

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